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CLLS1053

Sigma-Aldrich

MCF10A CELLS RICTOR (-/-)

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biological source

human female mammary glands (Source Disease: Fibrocystic Disease)

OMIM accession no.

storage temp.

−196°C

Gene Information

human ... RICTOR(253260)

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vibrant-m

CLLS1053

MCF10A CELLS RICTOR (-/-)

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CLLS1049

MCF10A CELLS TP53 (-/-)

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CLLS1059

MCF10A CELLS BCR (-/-)

vibrant-m

CLLS1042

MCF10A CELLS CDH1 (-/-)

OMIM accession no.

609022

OMIM accession no.

191170

OMIM accession no.

151410

OMIM accession no.

602118

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

storage temp.

−196°C

Gene Information

human ... RICTOR(253260)

Gene Information

human ... TP53(7157)

Gene Information

human ... BCR(613)

Gene Information

human ... CDH1(1105)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

MCF10A CELLS RICTOR (-/-) are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock out modification.

Application

Cell freezing medium-DMSO 1X, Cat. No. C6164
Media renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°
The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat No. H6909, to a final concentration of 0.5 ug/mL.

Features and Benefits

These MCF10A cells are adherent with a doubling time of approx. 20 hours
Zinc finger nuclease (ZFN) knockout on chromosome 5q13.1

Quality

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Kit Components Only

Product No.
Description

  • MCF10A CELLS RICTOR (-/-) 1 vial

Kit Components Also Available Separately

Product No.
Description
SDS

  • MCF10A CONTROL CELLS 1 vial

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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