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CMC0015

Sigma-Aldrich

BL21(DE3) pLysE Chemically Competent Cells

for protein expression

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NACRES:
NA.85

biological source

Escherichia coli

grade

for molecular biology

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

shipped in

dry ice

storage temp.

−70°C

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CMC0014CMC0016CMC0023
growth mode

adherent or suspension

growth mode

adherent or suspension

growth mode

adherent or suspension

growth mode

adherent or suspension

morphology

rod shaped

morphology

rod shaped

morphology

rod shaped

morphology

rod shaped

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

General description

The BL21(DE3) pLysE Chemically Competent Cells produce T7 lysozyme to repress low-level transcription from the T7 promoter prior to IPTG induction, helping to stabilize inserts coding for deleterious products.
These cells have a higher level of repression by T7 lysozyme, offering the greatest control over expression of inserts.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Features and Benefits

  • a transformation efficiency of >1 × 107 cfu/μg.
  • economical prices
  • offering superior value for everyday protein expression work.

Components

  • BL21(DE3) pLysE chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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Related Content

BL21 Chemically Competent Cells are provided in aliquots of 80 μL sufficient for two transformation reactions of 40 μL each. Transformation is performed by heat shock at 42 °C, followed by incubation on ice.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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