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CMR0001

Sigma-Aldrich

Recovery Medium for Expression

for use with competent cells

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NACRES:
NA.85

grade

for molecular biology

sterility

sterile; autoclaved

shipped in

dry ice

storage temp.

−20°C

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CMR0001

Recovery Medium for Expression

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pT7-FLAG-2 Expression Vector

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pRc/RSV Expression vector

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H8032

Hanahan′s Broth (SOB Medium)

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

shipped in

dry ice

shipped in

dry ice

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

room temp

General description

This recovery media has and optimized formulation for ensuring stability and high-efficiency transformation of protein expression clones.

Features and Benefits

  • Formulated without lactose to prevent unwanted leaky expression from T7 or other lactose/IPTG-inducible promoters.
  • Enables the highest possible transformation efficiency.
  • Use in the outgrowth stage of competent cell transformation to achieve 10-folder higher transformation efficiency than you would get with LB or SOC media.

Storage Class Code

10 - Combustible liquids


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Salvatore Di Girolamo et al.
Microbial cell factories, 19(1), 170-170 (2020-08-29)
Miniaturization of biochemical reaction volumes within artificial microcompartments has been the key driver for directed evolution of several catalysts in the past two decades. Typically, single cells are co-compartmentalized within water-in-oil emulsion droplets with a fluorogenic substrate whose conversion allows

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