This kit has not been validated for compatibility with formalin-fixed paraffin-embedded (FFPE) tissue sections. It is designed for cultured cells and advises against using ethanol- or methanol-based fixatives, as these can inactivate the β-galactosidase label critical for senescence detection. FFPE processing typically involves ethanol or reagent-grade alcohol (primarily ethanol with minor methanol and isopropyl alcohol components) during dehydration, which suggests potential incompatibility with the kit’s staining mechanism. Consequently, this kit is likely not suitable for FFPE-processed tissues without protocol optimization, e.g., alternative fixation methods or additional validation to ensure compatibility.
CS0030
Senescence Cells Histochemical Staining Kit
sufficient for 100 tests
Synonym(s):
Senescent cells IHC kit, cellular β-galactosidase probe
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1 KIT
MXP 11,266.00
MXP 11,266.00
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352207
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usage
sufficient for 100 tests
packaging
pkg of 1 kit
storage condition
dry at room temperature
detection method
colorimetric
shipped in
dry ice
storage temp.
−20°C
Quality Level
1 of 4
This Item | GALS | 12156792910 | 77730 |
|---|---|---|---|
| usage sufficient for 100 tests | usage kit sufficient for 100 tests (using a 3.5 cm dish) | usage sufficient for ≤50 tests | usage - |
| packaging pkg of 1 kit | packaging - | packaging - | packaging - |
| storage condition dry at room temperature | storage condition - | storage condition - | storage condition - |
| detection method colorimetric | detection method - | detection method - | detection method - |
| shipped in dry ice | shipped in dry ice | shipped in - | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. - |
General description
The Senescence Cells Histochemical Staining Kit contains all the reagents required for identifying senescent cells using an assay based on a histochemical stain for β-galactosidase activity at pH 6. β-galactosidase activity is detectable in senescent cells, but not in quiescent, immortal, or tumor cells. Cellular senescence is a progression of events, whereby cells move from an actively dividing to a non-dividing stage. The cellular senescence process is associated with aging. The decrease in cell division is virtually irreversible and complete. In conjunction with the loss of the ability to divide, changes occur in the morphology, shape, and physical appearance of the cells, and their pattern of gene expression. At the end of the process cell death usually occurs, although the cells may remain viable for a long time.
Application
Senescence Cells Histochemical Staining Kit has been used for:
- senescence-associated β-galactosidase assay
- to test for senescence in human pancreatic cancer cells
- adipose-derived stem cells (ADSCs)
- cord blood mesenchymal stromal/stem cells (CBMSC)
Biochem/physiol Actions
Replicative senescence is a growth-arrest state associated with loss of division potential, changes in cell morphology, shape and physical appearance, and the pattern of gene expression in cells.
Legal Information
Manufactured under license to US Patent Nos. 5,491,069 and 5,795,728.
Kit Components Only
Product No.
Description
- X-gal solution 4 mL
- Staining Solution, 10X 15 mL
- Fixation Buffer 10x 15 mL
- Reagent B 1.5 mL
- Reagent C 1.5 mL
- Dulbecco's Phosphate Buffered Saline (PBS) 10x 60 mL
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Description
Pricing
signalword
Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
supp_hazards
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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Mandy Oj Grootaert et al.
Autophagy, 11(11), 2014-2032 (2015-09-24)
Autophagy is triggered in vascular smooth muscle cells (VSMCs) of diseased arterial vessels. However, the role of VSMC autophagy in cardiovascular disease is poorly understood. Therefore, we investigated the effect of defective autophagy on VSMC survival and phenotype and its
Natalja E Fedorovich et al.
Biomaterials, 30(3), 344-353 (2008-10-22)
Photopolymerizable hydrogels, formed by UV-exposure of photosensitive polymers in the presence of photoinitiators, are widely used materials in tissue engineering research employed for cellular entrapment and patterning. During photopolymerization, the entrapped cells are directly exposed to polymer and photoinitiator molecules.
B van der Loo et al.
Experimental cell research, 241(2), 309-315 (1998-06-25)
A beta-galactosidase activity has recently been used as a histochemical marker of replicative senescence in human fibroblasts and keratinocytes. To establish whether this marker could be used to detect senescence of vascular cells, we have investigated its presence in cultures
G P Dimri et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(20), 9363-9367 (1995-09-26)
Normal somatic cells invariably enter a state of irreversibly arrested growth and altered function after a finite number of divisions. This process, termed replicative senescence, is thought to be a tumor-suppressive mechanism and an underlying cause of aging. There is
Phuc H Pham et al.
In vitro cellular & developmental biology. Animal, 53(1), 20-32 (2016-09-03)
As diseases and abnormalities of the heart can interfere with the aquaculture of Atlantic salmon, the heart was investigated as a source of cell lines that could be used to study the cellular basis of these conditions. An Atlantic salmon
Articles
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
Related Content
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Can this kit be used to stain FFPE sections?
1 answer-
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Can we use confocal/basic fluorescent microscope for capturing the expression of Beta Galactosidase after using this staining kit?
1 answer-
This kit has not been tested for use with confocal or fluorescent microscopes. X-gal staining does not involve a specific wavelength and it would be up to the end user to determine suitability. Note below, in a previously submitted question, that this kit has not been tested for dual staining with fluorescent dyes.
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Is there a protocol for staining HCHO-fixed tissue culture cells, specifically for cells growing on coverslips or slides?
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For staining purposes, there is no significant difference between staining wells attached to a culture plate or cells attached to a coverslip or glass slide. The primary difference lies in the amount of reagent required for the staining. It is advisable to ensure adequate coverage of the cells, and the insert contains a table that provides guidance on the amount of reagent required for staining.
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Does kit CS0030 include a counterstain?
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No, the CS0030 kit does not include a counterstain.
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Is there a method to extract the stain from cells when using the Senescence Cells Histochemical Staining Kit (Catalog Number CS0030) for cells in transwell inserts where cell counting is not feasible?
1 answer-
The CS0030 kit is intended for cell visualization using a microscope and does not include a specific procedure for dye extraction from cells.
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I have been using it well for a long time. thank you I want to stain beta-gal staining and apoptosis-related IF together. Is there a recommended order or method?
1 answer-
The CS0030 kit has not been tested for use in dual staining, it would be up to the end user to determine suitability. There are several factors to consider with regard to attempting this modification.
1. The CS0030 includes a fixation step. Cells are not viable at the time of staining. Therefore any 'live cell' fluorescent stain would not be suitable following fixation.
2. There is an incubation period of 2 hours to overnight. This may deplete or cause certain fluorescent dyes to fade which would mean a 'one-time use' for visualizing the IF-stained cells.
3. The staining mechanism for CS0030 is pH dependent. This may not be suitable for some fluorescent dyes.
4. One possible robust fluorescent stain to consider would be DAPI, which could be applied following the b-gal staining. DAPI will stain fixed cells and can be preserved if stored in the dark.To discuss further, please navigate to the link https://www.sigmaaldrich.com/techservice, and click on "Product Technical Inquiries" under the Products Section with all the required information so that a member of the Technical Service team can reach out to assist further.
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Can Product CS0030, Senescence Cells Histochemical Staining Kit be used with fixed cells/tissues?
1 answer-
Product CS0030, Senescence Cells Histochemical Staining Kit was designed for use with fresh (not-fixed) cells. The kit contains a fixation buffer to fix the cells. It is 10% formaldehyde, 1% glutaraldehyde in DPBS at 1X. The cells will be fixed prior to staining. The staining will show the beta-galactosidase activity that was present at the time of fixation.We tested the kit only on cells and we have no experience with tissue sections. The following papers use an assay for ßGAL on tissue sections which suggests that the kit will work with tissue sections.Castro P, et al., Interleukin-8 expression is increased in senescent prostatic epithelial cells and promotes the development of benign prostatic hyperplasia. Prostate, 60(2), 153-159 (2004).Chen, Z., et al., Crucial role of p53-dependent cellular senescence in suppression of Pten-deficient tumorigenesis. Nature, 436, 725-730 (2005).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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