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CS0390

Sigma-Aldrich

Mitochondria Staining Kit

1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)

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Synonym(s):
JC-1 dye
NACRES:
NA.32

Quality Level

usage

 kit sufficient for 200 tests (of 1 mL cell suspensions)
 kit sufficient for 40 tests (of 5 mL cell suspensions)

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

flow cytometry: suitable
protein staining: suitable

fluorescence

λex 490 nm; λem 530 nm (green) (JC-1 monomers)
λex 525 nm; λem 590 nm (red) (JC-1 aggregates)

application(s)

cell analysis
detection

detection method

fluorometric

shipped in

wet ice

storage temp.

2-8°C

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This Item
CS0760MAK160MAK159
Mitochondria Staining Kit 1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)

CS0390

Mitochondria Staining Kit

Isolated Mitochondria Staining Kit 1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

CS0760

Isolated Mitochondria Staining Kit

usage

 kit sufficient for 200 tests (of 1 mL cell suspensions)

usage

 kit sufficient for 1000 reactions (using 96 multiwell plates),  kit sufficient for 50 reactions (in a 2 mL cuvette)

usage

sufficient for 100 fluorometric tests (flow cytometry)

usage

sufficient for 500 fluorometric tests (microplate readers)

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

-

packaging

-

storage condition

dry at room temperature

storage condition

dry at room temperature

storage condition

-

storage condition

-

fluorescence

λex 490 nm; λem 530 nm (green) (JC-1 monomers)

fluorescence

λex 490 nm; λem 590 nm (JC-1 dye)

fluorescence

-

fluorescence

-

application(s)

cell analysis
detection

application(s)

cell analysis
detection

application(s)

-

application(s)

-

General description

In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) prevents the accumulation of the JC-1 dye in the mitochondria and thus, the dye is dispersed throughout the entire cell leading to a shift from red (JC-1-aggregates) to green fluorescence (JC-1 monomers). The fluorescence of the cells stained with this kit may be observed by fluorescence microscopy or measured by fluorimetric and flow cytometry analysis.

Application

The dissipation of the mitochondrial electrochemical potential gradient (Δψ) is known as an early event in apoptosis. This kit offers a fast and convenient method for the detection of changes in mitochondrial inner-membrane electrochemical potential in living cells using the cationic, lipophilic dye, JC-1.

Features and Benefits

Kit offers:
  • A fast, simple, and convenient method for staining and assaying mitochondria intactness
  • Contains all the reagents required for the detection of changes mitochondrial inner-membrane electrochemical potential
  • Contains the antibiotic valinomycin, which can be used as a control agent that prevents JC-1 aggregation
  • The shift in fluorescence is clearly detectable in both green and red channels
  • Suitable for adherent cells as well as cells in suspension
  • Has been tested with Jurkat, U-937, HeLa, NIH3T3 cells
  • The fluorescence signal can be observed by fluorescence microscopy or measured by flow cytometry

Kit Components Only

Product No.
Description

  • DMSO 1 mL

  • JC Staining Buffer 5X 120 mL

  • JC-1 1 mg

  • Valinomycin Ready Made .1 mL

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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A new method for the cytofluorimetric analysis of mitochondrial membrane potential in intact cells has been developed by using the lipophilic cationic probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1), whose monomer emits at 527 nm after excitation at 490 nm. Depending on the
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Oxidative stress is mediated, in part, by reactive oxygen species produced by multiple cellular processes and controlled by cellular antioxidant mechanisms such as enzymatic scavengers or antioxidant modulators. Free radicals, such as reactive oxygen species, cause cellular damage via cellular.

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