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D1529

Sigma-Aldrich

1-Fluoro-2,4-dinitrobenzene

≥99%

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Synonym(s):
2,4-Dinitro-1-fluorobenzene, DNFB, DNPF, FDNB, Sanger reagent
Linear Formula:
(O2N)2C6H3F
CAS Number:
Molecular Weight:
186.10
Beilstein:
398632
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.83

Quality Level

Assay

≥99%

form

solid

refractive index

n20/D 1.569 (lit.)

bp

178 °C/25 mmHg (lit.)

mp

25-27 °C (lit.)

density

1.482 g/mL at 25 °C (lit.)

storage temp.

room temp

SMILES string

[O-][N+](=O)c1ccc(F)c(c1)[N+]([O-])=O

InChI

1S/C6H3FN2O4/c7-5-2-1-4(8(10)11)3-6(5)9(12)13/h1-3H

InChI key

LOTKRQAVGJMPNV-UHFFFAOYSA-N

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This Item
4208573177D102504
1-Fluoro-2,4-dinitrobenzene for HPLC derivatization, LiChropur™, ≥99.0% (GC)

Supelco

73177

1-Fluoro-2,4-dinitrobenzene

form

solid

form

liquid

form

(Solid or Semi-Solid or Chunk(s) or Paste or Liquid)

form

powder

storage temp.

room temp

storage temp.

2-8°C

storage temp.

-

storage temp.

-

refractive index

n20/D 1.569 (lit.)

refractive index

n20/D 1.569 (lit.)

refractive index

n20/D 1.569 (lit.)

refractive index

-

bp

178 °C/25 mmHg (lit.)

bp

178 °C/25 mmHg (lit.)

bp

178 °C/25 mmHg (lit.)

bp

-

mp

25-27 °C (lit.)

mp

25-27 °C (lit.)

mp

25-27 °C (lit.)

mp

72-74 °C (lit.)

General description

1-Fluoro-2,4-dinitrobenzene (DNFB) is a reagent that modifies the N-terminal amino acids of proteins and peptides. DNFB is also commonly known as Sanger′s Reagent, named after Frederick Sanger, who reported in a classic 1945 paper the use of DNFB for derivatization of proteins and peptides specifically at their N-termini.†

Application

1-Fluoro-2,4-dinitrobenzene has been used to induce severe contact hypersensitivity in mice and to monitor gene expression by in vivo imaging method, and to induce chronic itch as a model for analysing itch-scratching behavior.

Biochem/physiol Actions

1-Fluoro-2,4-dinitrobenzene is a contact-sensitizing hapten, commonly used in experimental studies on contact hypersensitivity, inducing itch model, and atopic dermatitis. Mast cell activation is a key step in the DNFB induced sensitivity. DNFB covalently binds to the N-terminal amino acid of the protein and aids in Sanger sequencing.

Pictograms

Exclamation markHealth hazard

Signal Word

Warning

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

327.2 °F

Flash Point(C)

164 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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