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Merck

D2650

Sigma-Aldrich

Dimethyl sulfoxide

Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

Dimethyl sulfoxide

Synonym(s):

DMSO

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100 ML
MXP 6,011.00

MXP 6,011.00


Estimated to ship onJuly 14, 2025Details


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100 ML
MXP 6,011.00

About This Item

Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
Beilstein:
506008
EC Number:
MDL number:
UNSPSC Code:
12191502
PubChem Substance ID:
NACRES:
NA.75
Grade:
Hybri-Max
Technique(s):
cell culture | hybridoma: suitable
cryopreservation: suitable
Bp:
189 °C (lit.)
Vapor pressure:
0.42 mmHg ( 20 °C)

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MXP 6,011.00


Estimated to ship onJuly 14, 2025Details


Technical Service
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grade

Hybri-Max

Quality Level

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

sterility

sterile-filtered

product line

BioReagent

Assay

≥99.7%

form

liquid

autoignition temp.

573 °F

expl. lim.

42 %, 63 °F

technique(s)

cell culture | hybridoma: suitable
cryopreservation: suitable

impurities

endotoxin, tested
≤0.1% water
water

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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Show Differences

1 of 4

This Item
D2653D2438V900090
technique(s)

cell culture | hybridoma: suitable, cryopreservation: suitable

technique(s)

cell culture | hybridoma: suitable, cryopreservation: suitable

technique(s)

cell culture | hybridoma: suitable, cryopreservation: suitable

technique(s)

-

Quality Level

400

Quality Level

300

Quality Level

400

Quality Level

-

grade

Hybri-Max

grade

Hybri-Max

grade

BioPerformance Certified

grade

reagent grade

assay

≥99.7%

assay

≥99.7%

assay

-

assay

99%

solubility

H2O: miscible (completely)

solubility

H2O: miscible (completely)

solubility

H2O: miscible (completely)

solubility

-

form

liquid

form

liquid

form

liquid

form

liquid

Application

DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).

Packaging

100 mL in amber bottle
Glass ampoule packaging is now available under a new product number, D2653

Other Notes

This product is a Hybri-Max product. It is hybridoma tested and is assessed for suitability in cell freezing. This product is sterile filtered (0.2 micron) and tested for endotoxin levels.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

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Questions

1–10 of 20 Questions  
  1. Is this DMSO suitable to be used as a drug solvent in animal studies?

    1 answer
    1. This product has not been tested for use in animal models. However, this is a sterile-filtered DMSO that has been tested for cytotoxicity and is expected to be suitable for in vivo research applications.

      Helpful?

  2. How many days after opening the bottle is DMSO safe to use?

    1 answer
    1. This product is assigned an expiration date of approximately two years from the date of Quality Release. This date is lot specific and available on the product label and Certificate of Analysis. After opening, the material should be stored tightly sealed to prevent moisture uptake. Given proper handling and storage, the product should be stable until the published expiration date.

      Helpful?

  3. I ordered 10 x 10ml vials for fibroblast culture work. My first endeavor in culture domain. What are the storage conditions for DMSO of this grade? This arrived in plain cardboard box at room temperature.

    1 answer
    1. DMSO is hygroscopic and light-sensitive. The storage container must protect from light and moisture uptake. Since this DMSO is sterile-filtered and intended for cell culture applications, the storage containers must also be sterile and endotoxin-free. It is recommended to purge aliquots with inert gas and store them in a compatible, tightly sealed container, at a cool storage area, such as 4°C. DMSO is not compatible with many types of plastics, so it is advisable to store it in appropriate amber glass vials for long-term storage. Please refer to the Safety Data Sheet and the Product Information Sheet for compatibility of DMSO with various materials and for further details.

      Helpful?

  4. Is it safe in terms of health and product safety to store a leftover DMSO ampule covered with parafilm in a flammable cabinet?

    1 answer
    1. DMSO is hygroscopic and light-sensitive. The storage container must protect from light and moisture uptake. Since this DMSO is sterile-filtered and intended for cell culture applications, the storage containers must also be sterile and endotoxin-free. It is recommended to purge aliquots and store them under inert gas, in a compatible, tightly sealed container, at a cool storage area, such as 4°C.

      DMSO is not compatible with many types of plastics, so it is advisable to store it in appropriate amber glass vials for long-term storage. Please refer to the Product Information Sheet and the Safety Data Sheet at the link below for compatibility of DMSO with various materials and for further details:

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/944/265

      Helpful?

  5. Is this anhydrous?

    1 answer
    1. This product is not considered anhydrous. The maximum allowable water content is 0.1% which is reported on the lot-specific Certificate of Analysis. Please see the link below to review the anhydrous DMSO products available:
      https://www.sigmaaldrich.com/search/dmso-anhydrous?focus=products&page=1&perpage=30&sort=relevance&term=DMSO%20anhydrous&type=product

      Helpful?

  6. I ordered 10 ml glass vials, used 7 ml, Can I store it in centrifuge tubes? For how long can I use that?

    1 answer
    1. It may be best to store DMSO in amber glass vials for long term storage, as this compound is not compatible with many types of plastics. The compatibility of DMSO with various materials is listed below:
      Compatible: LDPE, HDPE, polypropylene, PPCO(polypropylene copolymer), polymethylpentene, nylon, teflon FEP
      Moderately compatible: polystyrene, ECTFE/ETFE
      Incompatible: polysulfone, flexible and rigid PVC tubing, polycarbonate

      Note that DMSO is hygroscopic and light sensitive. The storage container selected must offer protection from light and moisture uptake. The product is assigned a firm expiration date. Please refer to the lot specific Certificate of Analysis to assume the material is within the expected shelf life.

      Helpful?

  7. E' solo ad uso per ricerca o utilizzabile anche in diagnostica/human? Grazie

    1 answer
    1. This product is not suitable for diagnostic/human use.

      Helpful?

  8. Is this product packaged in a bottle with a septa cap?

    1 answer
    1. No, this product is not packaged with a septa cap. It comes in a 125 ml amber glass bottle with a 28 mm solid white cap.

      Helpful?

  9. Can I use it for PCR?

    1 answer
    1. While this product is tested specifically for use in cell culture, there is no reason to suspect that it would not perform well in PCR applications. The product is NOT tested for amplification, DNAse, or nuclease activity. Should this testing be important, see the link below to product options that include this testing:
      D9170 DMSO PCR Reagent:
      https://www.sigmaaldrich.com/product/sigma/d9170
      D8418 DMSO Molecular Biology Tested
      https://www.sigmaaldrich.com/US/en/product/sigma/d8418

      Helpful?

  10. what is the recommended re-test date & for how long can it be used beyond the expiry date mentioned in the COA?

    1 answer
    1. This product is assigned a firm expiration date of approximately 2 years. It is not assigned a retest date. The stability beyond the lot-specific expiration date is not determined and the quality of the product cannot be guaranteed. See the link below to review a sample Certificate of Analysis: https://www.sigmaaldrich.com/coa/SIGMA/D2650/RNBK5742.

      Helpful?

1–10 of 20 Questions  

Reviews

Active Filters

  1. Turkey
    • Review 1
    • Votes 7
    5 out of 5 stars.

    Is this product already concentration adjusted

    Is this product already concentration adjusted, as you mentioned this product used %8.7 concentration bu the thing i cannot understand that is this item already diluted for ready to use or do we have to dilute it what concentration we need ?

    Helpful?

  2. 9 Ratings-only reviews

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