D2915

Sigma-Aldrich

Dexamethasone-Water Soluble

suitable for cell culture, BioReagent

Synonym(s):
Dexamethasone – Cyclodextrin complex
CAS Number:
EC Number:
PubChem Substance ID:
NACRES:
NA.77

Quality Level

biological source

synthetic (organic)

product line

BioReagent

form

solid

application(s)

cell culture | mammalian: suitable

solubility

H2O: 25 mg/mL, clear to slightly hazy

shipped in

ambient

storage temp.

room temp

SMILES string

C[C@@H]1CC2C3CCC4=CC(=O)C=C[C@]4(C)C3[C@@H](O)C[C@]2(C)[C@H]1C(=O)CO.OC[C@H]5O[C@@H]6O[C@H]7[C@H](O)[C@@H](O)[C@H](O[C@@H]7CO)O[C@H]8[C@H](O)[C@@H](O)[C@H](O[C@@H]8CO)O[C@H]9[C@H](O)[C@@H](O)[C@H](O[C@@H]9CO)O[C@H]%10[C@H](O)[C@@H](O)[C@H](O[C@@H]%10CO)O[C@H]%11[C@H](O)[C@@H](O)[C@H](O[C@@H]%11CO)O[C@H]%12[C@H](O)[C@@H](O)[C@H](O[C@@H]%12CO)O[C@H]5[C@H](O)[C@H]6O

InChI

1S/C42H70O35.C22H30O4/c43-1-8-29-15(50)22(57)36(64-8)72-30-9(2-44)66-38(24(59)17(30)52)74-32-11(4-46)68-40(26(61)19(32)54)76-34-13(6-48)70-42(28(63)21(34)56)77-35-14(7-49)69-41(27(62)20(35)55)75-33-12(5-47)67-39(25(60)18(33)53)73-31-10(3-45)65-37(71-29)23(58)16(31)51;1-12-8-16-15-5-4-13-9-14(24)6-7-21(13,2)20(15)17(25)10-22(16,3)19(12)18(26)11-23/h8-63H,1-7H2;6-7,9,12,15-17,19-20,23,25H,4-5,8,10-11H2,1-3H3/t8-,9-,10-,11-,12-,13-,14-,15-,16-,17-,18-,19-,20-,21-,22-,23-,24-,25-,26-,27-,28-,29-,30-,31-,32-,33-,34-,35-,36-,37-,38-,39-,40-,41-,42-;12-,15+,16?,17+,19-,20-,21+,22+/m11/s1

InChI key

XEJNDGTUKUMQNG-ARJMFYCQSA-N

Related Categories

Application

Dexamethasone-Water Soluble has been used as a component of
  • Dulbecco′s modified Eagle medium based osteogenic and adipogenic medium for human mesenchymal stem cell culture
  • erythroid expansion media for the growth of liver, bone marrow and spleen tissues
  • medium 199 (M199) for hepatocyte culture

Packaging

100 mg in glass bottle
Package size based on dexamethasone

Biochem/physiol Actions

Dexamethasone is an anti-inflammatory glucocorticoid and a synthetic corticosteroid used for treating corneal inflammation. It inhibits the IL-1β-dependent angiogenesis. Dexamethasone elicits a range of effects on cell survival, cell signaling and gene expression. It favors apoptosis in chondrocytes and impairs development of bone. Dexamethasone is a potential therapeutic agent as it induces apoptosis in multiple myeloma (MM) cells. It elicits its functionality by lowering glucocorticoid receptor (GR) α levels and inhibits colon tumor progression.

Other Notes

Contains approx. 65 mg dexamethasone per gram; balance methyl-β-cyclodextrin.

pictograms

Health hazard

signalword

Danger

hcodes

hazcat

Repr. 1B

storage_class_code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

  1. What cell lines has Product D4902, Dexamethasone, been tested on?

    The cell lines on which this product is tested can vary from lot to lot. Some typically used cell lines are 3T3 fibroblasts, BHK, L929 and CHO.

  2. Are Products D4902 and D2915, sterile?

    No. This product is not sterile, but cell culture testing did not find the product to be toxic to cells.

  3. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  4. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  5. How long is Product D2915, Dexamethasone-Water Soluble, stable in solution?

    Dexamethasone solutions are stable for 6 months when aliquoted stored at -20 °C in a non-defrosting freezer and protected from light.  However, insufficient information is available to assess the shelf-life of solutions stored at 4°C.

  6. What is the molecular weight of Product D2915, Dexamethasone-Water Soluble?

    The molecular weight of dexamethasone alone is 392.5 g/mol. Be sure to take the weight of the carrier into account for any calculations involving molarity of solutions.

  7. How much Product D2915, Dexamethasone-Water Soluble, have I received?

    Each vial contains 100mg of Dexamethasone.  The milligrams of Dexamethasone per gram (Dexamethasone content) is listed on the Certificate of Analysis, which can be accessed through our Advanced Search Option.This information can be used to determine the conversion factor to make a solution of a given molarity.

  8. Can I use Product D2915, Dexamethasone-Water Soluble, to inject into mice?

    We do not perform animal studies with our products, but it can be used in cell culture.

  9. How should I reconstitute Product D2915, Dexamethasone-Water Soluble, for tissue culture use?

    The tissue culture grade product is tested in water at a concentration of 25 mg/mL forming a clear to slightly hazy colorless solution.

  10. Do you have references for the use of Product D2915, Dexamethason - Water Soluble?

    Here is one reference that describes using Product No. D2915: Chang, C.-J., et al., Coactivator TIF1? Interacts with Transcription Factor C/EBP? and Glucocorticoid Receptor To Induce ?1-Acid Glycoprotein Gene Expression. Mol. Cell. Biol., 18(10), 5880-5887 (1998).

Plasma membrane phospholipase A2 controls hepatocellular fatty acid uptake and is responsive to pharmacological modulation: implications for nonalcoholic steatohepatitis
Stremmel W, et al.
Faseb Journal, 28(7), 3159-3170 (2014)
Dexamethasone inhibits interleukin-1beta-induced corneal neovascularization: role of nuclear factor-kappaB-activated stromal cells in inflammatory angiogenesis
Nakao S, et al.
The American Journal of Pathology, 171(3), 1058-1065 (2007)
Immature erythroblasts with extensive ex vivo self-renewal capacity emerge from the early mammalian fetus
England SJ, et al.
Blood, 117(9), 2708-2717 (2011)
Dexamethasone-induced apoptotic mechanisms in myeloma cells investigated by analysis of mutant glucocorticoid receptors
Sharma S and Lichtenstein A
Blood, 112(4), 1338-1345 (2008)
Glucocorticoid (dexamethasone)-induced metabolome changes in healthy males suggest prediction of response and side effects
Bordag N, et al.
Scientific Reports, 5, 15954-15954 (2015)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service