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D4904

Sigma-Aldrich

pBR322 Plasmid DNA from E. coli RRI

buffered aqueous solution

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CAS Number:
EC Number:
MDL number:
eCl@ss:
32160414

recombinant

expressed in E. coli

Quality Level

grade

for molecular biology

form

buffered aqueous solution

mol wt

2.9 MDa
4363 bp

Origin of replication

BR322

selection

ampicillin

shipped in

dry ice

storage temp.

−20°C

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This Item
D9893D3404D4154
grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

grade

for molecular biology

form

buffered aqueous solution

form

lyophilized powder

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

2.9 MDa, 4363 bp

mol wt

2.9 MDa, 4363 bp

mol wt

-

mol wt

-

origin of replication

BR322

origin of replication

-

origin of replication

-

origin of replication

-

shipped in

dry ice

shipped in

-

shipped in

dry ice

shipped in

dry ice

General description

Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in E. coli. This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives. Plasmid pBR322 confers resistance to ampicillin and tetracycline. The plasmid sequence has been published.

The plasmid has unique restriction sites within the gene for ampicillin resistance (Pst I, Pvu I, and Sca I), within the gene for tetracycline resistance (BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, and Xma III), and elsewhere (Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, and Tth111 I).

Specificity

Unique Sites: Within the gene for ampicillin resistance: Pst I, Pvu I, Sca I; Within the gene for tetracycline resistance: BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, Xma III; other unique sites: Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, Tth111 I.

Application

Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in Escherichia coli. This plasmid and derivatives have been used for a number of purposes including cloning, selection and expression of recombinant molecules, construction of shuttle vectors and vectors for nucleotide sequencing, studies of elements involved in gene expression, as plasmid DNA standards, and as a model system for studies on prokaryotic plasmid replication.
pBR322 Plasmid DNA from E. coli RRI has been used to study about ferrous ion-induced strand breaks in the plasmid pBR322 are mediated through hydrogen peroxide.

Biochem/physiol Actions

This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives.

Components

DNA is provided in a solution of 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Other Notes

Accession number: J01749

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Jörg Flemmig et al.
European biophysics journal : EBJ, 36(4-5), 377-384 (2006-10-19)
Ferrous ion-induced generation of single and multiple strand breaks in the DNA plasmid pBR322 induces the formation of two new plasmid forms with altered electrophoretic mobility. The yield of these plasmid forms, the circular relaxed and the linear forms, depended
Ferrous ion-induced strand breaks in the DNA plasmid pBR322 are not mediated by hydrogen peroxide
Flemmig J and Arnhold J
European Biophysics Journal, 36(4-5), 377-384 (2007)
Charles J Addison et al.
BioTechniques, 37(3), 376-378 (2004-10-09)
Transformation of Escherichia coli plays an important role in recombinant DNA technology. Most current transformation protocols require that the cells be treated to attain a particular physiological state known as "competence," and this makes transformation procedures lengthy and arduous. Here

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