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D9893

Sigma-Aldrich

pBR322 Plasmid DNA from E. coli RRI

lyophilized powder

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CAS Number:
EC Number:
MDL number:
eCl@ss:
32160414
NACRES:
NA.52

biological source

Escherichia coli

Quality Level

grade

for molecular biology

form

lyophilized powder

mol wt

2.9 MDa
4363 bp

storage temp.

2-8°C

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This Item
D4904D3404D4154
form

lyophilized powder

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

2.9 MDa, 4363 bp

mol wt

2.9 MDa, 4363 bp

mol wt

-

mol wt

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

biological source

Escherichia coli

biological source

-

biological source

-

biological source

Escherichia coli (RRI)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in E. coli. This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives. Plasmid pBR322 confers resistance to ampicillin and tetracycline. The plasmid sequence has been published.

The plasmid has unique restriction sites within the gene for ampicillin resistance (Pst I, Pvu I, and Sca I), within the gene for tetracycline resistance (BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, and Xma III), and elsewhere (Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, and Tth111 I).

Specificity

Unique Sites: Within the gene for ampicillin resistance: Pst I, Pvu I, Sca I; Within the gene for tetracycline resistance: BamH I, BspM I, EcoR V, Nhe I, Nru I, Sal I, Sph I, Xma III; other unique sites: Aat II, Ava I, Bal I, Bsm I, BspM II, Cla I, EcoR I, Hind III, Nde I, Pvu II, Ssp I, Sty I, Tth111 I.

Application

Plasmid pBR322 was one of the first multipurpose cloning vectors constructed for use in Escherichia coli. This plasmid and derivatives have been used for a number of purposes including cloning, selection and expression of recombinant molecules, construction of shuttle vectors and vectors for nucleotide sequencing, studies of elements involved in gene expression, as plasmid DNA standards, and as a model system for studies on prokaryotic plasmid replication.
pBR322 Plasmid DNA from E. coli RRI has been used as a low molecular weight DNA to test the enzymatic activity of partially purified component from Perna viridis (Linn.) It has also been used for investigating its effect on the calcium carbonate particle morphology by scanning electron microscopy studies

Biochem/physiol Actions

This plasmid is derived from the ColE1-type plasmid pMB1 and shares the same type of replication mechanism and controls as ColE1 and relatives.

Components

DNA is provided as a powder, lyophilized from a solution of 1 mM Tris-HCl (pH 7.5) with 1mM NaCl and 1mM EDTA.

Other Notes

Accession number: J01749
Accession number: J01749

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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