For monolayer culture or when a thin coating of BME is desired, dilutions of as high as 1:100 can be used. An empirically determined dilution should be performed depending on the particular application. Human embryonic stem cells and keratinocytes grow well when cultured on a 1:100 dilution of MaxGel ECM.
Please follow the instructions from the Product Information Sheet linked below:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/243/108/e0282dat.pdf
E0282
MaxGel™ ECM
mixture, liquid
Synonym(s):
Human Basement Membrane Extract (BME)
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1 ML
MXP 2,828.00
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1 ML
MXP 2,828.00
About This Item
UNSPSC Code:
12352207
eCl@ss:
32190102
NACRES:
NA.71
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Application
MaxGel™ ECM is used for the following applications:
This product creates a three-dimensional environment that improves cellular proflieration by promoting cell growth and migration, and has been shown to aid the proliferation of many types of cells, including human embryonic stem cells, neural stem cells, neurons, glia, astrocytes, fibroblasts, hepatocytes, and keratinocytes.
Components
MaxGel ECM contains human extracellular matrix components including collagens, laminin, fibronectin, tenascin, elastin and a number of proteoglycans and glycosaminoglycans. This basement membrane extract reproduces the cooperative interactions between epithelia and mesenchyme that occur during the development and organotypic cell culture of skin.
Caution
MaxGel will remain stable for at least 1 year when stored at -70°C.
Legal Information
MaxGel is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
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Hello there, Could you please provide the reconstitution details of this item(cat# E0282), I would like to know the ratios for example is it 1:100 etc.
1 answer-
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How do I handle Product E0282, MaxGel™ ECM?
1 answer-
Remove the product from the freezer and thaw on ice just prior to use. Keep the product cold until coating.
Please see the link below to review the product datasheet which includes the coating protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/243/108/e0282dat.pdf
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is the Product E0282, MaxGel™ ECM from normal tissue or tumor tissue?
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This product is derived from the basement membranes of epithelial cells and fibroblasts. These are not strictly tumor cell lines as the normal genotype and phenotype are retained.
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Can this product also be used for cell differentiation?
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The impact of extracellular matrix on cell differentiation can vary from cell type to cell type. This product is derived from human basement membrane extract that includes several components, many of which are not defined. Cell differentiation may be induced by the structural environment or other components present within the matrix. The end user would have to determine the suitability of the product based on the cell or tissue type of interest.
Please refer to the literature for additional information. The following link may be of interest:
https://biosignaling.biomedcentral.com/articles/10.1186/s12964-023-01252-8#:~:text=Several%20studies%20have%20demonstrated%20that,a%20certain%20niche%20%5B46%5D.Please see the link below to review the product datasheet for this item:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/243/108/e0282dat.pdfHelpful?
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How is Product E0282, MaxGel™ ECM, supplied?
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E0282 is supplied at a concentration of 1 mg/ml in DMEM.
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Will Product E0282, MaxGel™ ECM, work for adherent cells and suspension cells?
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Yes, this product works for both cell types.
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What is an appropriate working concentration for Product E0282, MaxGel™ ECM?
1 answer-
MaxGel™ works best undiluted or even diluted 1:2-1:4. For a thin coat plating method, this material can be diluted as high as 1:100.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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