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Monoclonal ANTI-FLAG® M2 antibody produced in mouse

1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Anti-ddddk, Anti-dykddddk, flag antibody

Quality Level

biological source




antibody form

affinity isolated antibody

antibody product type

primary antibodies


M2, monoclonal


buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

purified by

affinity chromatography (affinity tag purification)

species reactivity



1 mg/mL



immunogen sequence


shipped in

wet ice

storage temp.


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General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Method of purification - Affinity tag purification


Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C


FLAG; peptide sequence DYKDDDDK


For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) , immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Western Blotting and EIA

Browse additional application references in our FLAG® Literature portal.

Physical form

Contains 50% glycerol in phosphate buffered saline.

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Monika Srivastava et al.
Nature communications, 6, 6253-6253 (2015-02-24)
Roquin is an RNA-binding protein that prevents autoimmunity and inflammation via repression of bound target mRNAs such as inducible costimulator (Icos). When Roquin is absent or mutated (Roquin(san)), Icos is overexpressed in T cells. Here we show that Roquin enhances
HSV-2 glycoprotein gD targets the CC domain of tetherin and promotes tetherin degradation via lysosomal pathway
Yalan Liu
Virology, 154-154 (2016)
ADAR2 regulates RNA stability by modifying access of decay-promoting RNA-binding proteins
Aparna A
Nucleic Acids Research, 45(7), 4189-4201 (2017)
Histone H1 Limits DNA Methylation in Neurospora crassa
Michael Seymour
G3 (Bethesda, Md.) (2016)
Yumin Qiu et al.
Biochimica et biophysica acta. Molecular cell research, 1866(9), 1412-1420 (2019-06-10)
Src is a known proto-oncogene and its aberrant activity is involved in a variety of cancers, including ovarian cancer, whereas the regulatory mechanism of Src has not been fully clarified. In this study, we identified tripartite motif-containing (TRIM) 50 as


Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

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