All Photos(1)



Anti-Vinculin−FITC antibody, Mouse monoclonal

clone hVIN-1, purified from hybridoma cell culture

Monoclonal Anti-Vinculin antibody produced in mouse
MDL number:

Quality Level

biological source



FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies


hVIN-1, monoclonal


buffered aqueous solution

mol wt

antigen 116 kDa

species reactivity

bovine, canine, mouse, rat, turkey, human, chicken, frog

storage condition

protect from light


direct immunofluorescence: 1:50 using methanol/acetone fixed cultured bovine MDBK cells



UniProt accession no.

shipped in

dry ice

storage temp.


Gene Information

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

Looking for similar products? Visit Product Comparison Guide


Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.


purified human vinculin from uterus.


Monoclonal Anti-Vinculin-FITC antibody is suitable for immunofluorescence staining of fibroblasts cells to study the effects of nanofiber spatial organization on cell behaviors. It is also suitable for direct immunofluorescence at a dilution of 1:50 using methanol/acetone fixed cultured bovine MDBK cells.

Biochem/physiol Actions

Vinculin is a cytoskeletal protein with molecular mass of ~116 kDa. Vinculin is closely associated with adhesive membrane areas, such as focal contacts, podosomes, or intercellular junctions of the zonula adherens type. It acts as a mediator in interactions of integrins and the actin cytoskeleton. It forms a bridge between integrins, receptors for ECM molecules on the cell surface and the F-actin cytoskeleton. It is associated with talin and α-actinin which directly connects with integrins. It has ability to bind α-catenin as well as the vasodilator stimulated phosphoprotein (VASP) in focal contact assembly.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).


Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service