F8424

Fibroblast Growth Factor-4 human

FGF-4, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): hFGF-4, FGF-4, K-FGF
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00675383
• Biological source: human
• Recombinant: expressed in E. coli
• Assay: ≥95% (SDS-PAGE)
• Form: lyophilized powder
• Mol wt: monomer 19 kDa ((177 aa))
• Impurities: <1 EU/μg (LAL test)

Properties

• Product Name: Fibroblast Growth Factor-4 human, FGF-4, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• biological source: human
• recombinant: expressed in E. coli
• form: lyophilized powder
• potency: ≤10.0 ng/mL
• quality: endotoxin tested
• packaging: pkg of 25 and 100 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: P08620
• storage temp.: −20°C
• Quality Level: 200
• Gene Information: human ... FGF4(2249), FGF4(2249)
• assay: ≥95% (SDS-PAGE)
• mol wt: monomer 19 kDa ((177 aa))
• impurities: <1 EU/μg (LAL test)

Description

Application

Fibroblast Growth Factor-4 human has been used:

• To maintain trophectoderm stem cells.[1]
• In neurogenic differentiation.[2]
• In hepatocyte differentiation.[3]

Biochem/physiol Actions

Fibroblast growth factor-4 (FGF-4) is a 19 kDa protein identified in NIH-3T3 cell assays as a transforming gene from DNA derived from human tumors, including stomach (hst) and colon cancers, Kaposi′s sarcoma, and hepatocellular carcinoma. FGF-4 includes a secretory signal sequence and shares 42% sequence identity with FGF-Basic at the amino acid level. Both FGF-4 and FGF-Basic bind to the same receptors. Mouse and human FGF-4 shares 82% homology with species cross-reactivity. FGF-4 does not appear to be expressed in normal adult tissues, but during embryogenesis, the gene is spatially and temporally regulated. FGF-4 is a mitogen for fibroblasts and endothelial cells and a potent promoter of angiogenesis. It is believed to be critical in embryonic limb development.

Fibroblast growth factor-4 (FGF-4) is identified in NIH-3T3 cell assays as a transforming gene from DNA derived from human tumors, including stomach (hst) and colon cancers, Kaposi′s sarcoma, and hepatocellular carcinoma. FGF-4 includes a secretory signal sequence and shares 42% sequence identity with FGF-Basic at the amino acid level. Both FGF-4 and FGF-Basic bind to the same receptors. Mouse and human FGF-4 shares 82% homology with species cross-reactivity. FGF-4 does not appear to be expressed in normal adult tissues, but during embryogenesis, the gene is spatially and temporally regulated. FGF-4 is a mitogen for fibroblasts and endothelial cells and a potent promoter of angiogenesis. It is believed to be critical in embryonic limb development. FGF-4 participates in cell differentiation and cell migration.[4]

General description

Fibroblast Growth Factor-4 (FGF4) is a 19 kDa protein, belonging to the FGF family of proteins.[4] Fibroblast growth factors (FGF) are glycoproteins concealed in the extracellular matrix and cell surface.[5] There are around 22 different types of FGFs known. These factors weigh in the range of 17 to 34 kDa and they possess a conserved sequence of 120 amino acids.[6] The FGF4 gene is mapped to human chromosome 11q13.3.[7]

Analysis Note

The biological activity is measured by its ability to stimulate NR6R-3T3 cell proliferation.

Physical form

Lyophilized from a 0.2 µm filtered solution containing 20 mM Sodium Phosphate, 0.5 M Sodium Chloride, pH 7.5

Preparation Note

Reconstitution: Sterile water at 0.1 mg/mL

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)