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G1401

Sigma-Aldrich

Glial Cell Line-derived Neurotrophic Factor from rat

recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture, ≥97% (SDS-PAGE)

Synonym(s):
ATF, GDNF, Astrocyte-derived trophic factor
MDL number:
NACRES:
NA.32

Quality Level

biological source

rat

recombinant

expressed in baculovirus infected Sf21 cells

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

0.10-10 ng/mL

mol wt

~30 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

rat ... Gdnf(25453)

Biochem/physiol Actions

Glial cell-derived neurotrophic factor (GDNF) is a neurotrophic factor that is a member of the TGF-β superfamily. GDNF is founding member of the GDNF family of ligands, which to date include GDNF, neurturin (NTN), persephin (PSP) and artemin (ART). GDNF is a glycosylated disulfide-linked homodimeric protein of ~15 kDa. Mature rat and human GDNF share 93% sequence homology with strong species cross-reactivity. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development including motoneurons, midbrain dopaminergic neurons, Purkinje cells, and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes, and skeletal muscle cells. In addition, exogenously applied GDNF has been shown to rescue damaged facial motor neurons in vivo.

Physical form

Lyophilized from 50 μL of a 0.2 um filtered solution in PBS, pH 7.4 with 50 ug BSA per 1 ug as a carrier protein

Analysis Note

The biological activity of GDNF is measured by its ability to bind to immobilized rrGFRa1/Fc in a functional ELISA.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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