Merck
All Photos(1)

G6784

Sigma-Aldrich

L-Glutamine–Penicillin–Streptomycin solution

L-glutamine 200 mM, penicillin 10,000 units, streptomycin 10 mg/mL, 0.1 μm filtered, BioReagent, suitable for cell culture

Synonym(s):
Anti-TUBB4, Anti-FEOM3
MDL number:
NACRES:
NA.76

Quality Level

biological source

synthetic

description

stabilized

sterility

0.1 μm filtered

product line

BioReagent

form

liquid

composition

L-glutamine, 200 mM
penicillin, 10,000 units
streptomycin, 10 mg/mL

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

Mode of action

cell wall synthesis | interferes
protein synthesis | interferes

storage temp.

−20°C

Related Categories

Application

This antibiotic solution is used to reduce or eliminate bacterial contamination in primary cell cultures. It is recommended for us at 10 mL/L.

Biochem/physiol Actions

Mode of Action: The product acts in three ways: Glutamine is an essential energy source for most mammalian cells in culture due to its amino acid carbon skeleton. Penicillin inhibits bacterial cell wall synthesis. Streptomycin inhibits prokaryote protein synthesis by preventing the transition from initiation complex to chain-elongating ribosome, causing miscoding.

Antimicrobial spectrum: This product acts against both gram-negative and gram-positive bacteria.

Components

This solution is formulated to contain 200 mM L-glutamine, 10,000 units of Penicillin and 10 mg/mL of streptomycin, solubilized in a proprietary buffer.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Articles

L-Glutamine in Cell Culture

Importance and uses of glutamine in hybridoma and mammalian cell culture

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service