All Photos(3)

G8898

Sigma-Aldrich

Glycine

suitable for electrophoresis, ≥99%

Synonym(s):
Aminoacetic acid, Aminoethanoic acid, Glycocoll
Linear Formula:
NH2CH2COOH
CAS Number:
Molecular Weight:
75.07
Beilstein:
635782
EC Number:
MDL number:
eCl@ss:
32160406
PubChem Substance ID:

Quality Level

assay

≥99%

form

powder

application(s)

electrophoresis: suitable
western blot: suitable

impurities

≤0.01% Insoluble matter

color

white to off-white

pKa (25 °C)

(1) 2.35, (2) 9.60
2.35

mp

240 °C (dec.) (lit.)

solubility

H2O: 200 mg/mL, clear, colorless to faintly yellow

anion traces

chloride (Cl-): ≤70 ppm

cation traces

heavy metals (as Pb): ≤20 ppm

absorption

≤0.15 at 280 at 1 M

SMILES string

NCC(O)=O

InChI

1S/C2H5NO2/c3-1-2(4)5/h1,3H2,(H,4,5)

InChI key

DHMQDGOQFOQNFH-UHFFFAOYSA-N

Gene Information

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General description

Glycine is a molecule with pI of 6.7, which is similar to the pH of stacking gel. It has advantage of low mobility, hydrophobicity and it does not associate with proteins. Glycine is a component of Tris-glycine and Tris-glycine-SDS (sodium dodecyl sulfate) running buffers for polyacrylamide gel electrophoresis. Glycine is also a component of Towbin′s transfer buffer for Western blots.

Application

  • Glycine has been used for the preparation of gelatin composites scaffold.
  • It has been used in the elution buffer to extract protein using HiTrap protein G column.
  • It has been used in the SDS-sample, running and transfer buffers prepared for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and western blotting.

Packaging

500 g in poly bottle
1 kg in poly bottle

Biochem/physiol Actions

Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Analysis Note

Tested for use in electrode buffers for PAGE and in transfer buffers for Western blots.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Quantitative immunofluorescent blotting of the multidrug resistance-associated protein 2 (MRP2).
Gerk PM
Journal of Pharmacological and Toxicological Methods, 63, 279-279 (2011)
The role of muscle-derived stem cells in bone tissue engineering.
Sun JS et al.
Biomaterials, 26, 3953-3953 (2005)
Hames, B.D. and Rickwood, D.
Gel Electrophoresis of Proteins: A Practical Approach, 32-35 (1990)
Westermeier R
Electrophoresis in Practice: A Guide to Methods and Applications of DNA and Protein Separations (2016)
H Towbin et al.
Proceedings of the National Academy of Sciences of the United States of America, 76(9), 4350-4354 (1979-09-01)
A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. The method results in quantitative transfer of ribosomal proteins from gels containing urea. For sodium dodecyl sulfate gels, the original band pattern was...

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