G9785

GSEM Supplement

50 ×, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): Glutamine synthetase expression medium supplement
• MDL number: MFCD00677096
• NACRES: NA.75

Properties

• sterility: sterile-filtered
• Quality Level: 200
• form: liquid
• concentration: 50 ×
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Glutamine synthetase expression medium (GSEM) supplement enables formation of glutamine from ammonia and glutamate there by alleviating accumulation of ammonia in culture medium. It is exploited for high yield recombinant protein production in Chinese hamster ovary (CHO) cells.

Application

GSEM Supplement has been used as a supplement for ProCHO5 Protein-free medium for culturing Chinese hamster ovary (CHO) cells and selective medium for NS0 myeloma cells.

Packaging

100 mL

Contains (mg/L): L-alanine 450.0, L-asparagine · H₂O 4261.0, L-aspartic acid 650.0, L-glutamic acid 3750.0, L-proline 575.0, L-serine 500.0, adenosine 350.0, guanosine 350.0, cytidine 350.0, uridine 350.0, and thymidine 12.0

Quantity

One mL will supplement 50 mL of cell culture medium.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable