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H0135

Sigma-Aldrich

Hydrocortisone

γ-irradiated, powder, BioXtra, suitable for cell culture

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Synonym(s):
11β,17α,21-Trihydroxypregn-4-ene-3,20-dione, 17-Hydroxycorticosterone, 4-Pregnene-11β,17α,21-triol-3,20-dione, Cortisol, Kendall’s compound F, Reichstein’s substance M
Empirical Formula (Hill Notation):
C21H30O5
CAS Number:
Molecular Weight:
362.46
Beilstein:
1354819
EC Number:
MDL number:
PubChem Substance ID:

biological source

synthetic (organic)

Quality Level

sterility

γ-irradiated

product line

BioXtra

Assay

≥80% (HPLC)

form

powder

technique(s)

cell culture | mammalian: suitable

mp

211-214 °C (lit.)

solubility

ethanol: 1 mg/mL, clear

shipped in

ambient

storage temp.

room temp

SMILES string

[H][C@@]12CCC3=CC(=O)CC[C@]3(C)[C@@]1([H])[C@@H](O)C[C@@]4(C)[C@@]2([H])CC[C@]4(O)C(=O)CO

InChI

1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1

InChI key

JYGXADMDTFJGBT-VWUMJDOOSA-N

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This Item
PHR1014H0888H6909
Hydrocortisone γ-irradiated, powder, BioXtra, suitable for cell culture

H0135

Hydrocortisone

Hydrocortisone Pharmaceutical Secondary Standard; Certified Reference Material

PHR1014

Hydrocortisone

Hydrocortisone BioReagent, suitable for cell culture

H0888

Hydrocortisone

Hydrocortisone solution 50 μM, sterile-filtered, BioXtra, suitable for cell culture

H6909

Hydrocortisone solution

assay

≥80% (HPLC)

assay

-

assay

≥98%

assay

-

product line

BioXtra

product line

-

product line

BioReagent

product line

BioXtra

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

500

technique(s)

cell culture | mammalian: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

-

form

powder

form

liquid

General description

Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.

Application

Hydrocortisone is used for the following applications:
  • Used in myogenic induction and identification
  • Used in long-term stromal cultures and methylcellulose cultures (one of the components in the culture medium)
  • Used in adipogenic differentiation (one of the components in adipogenic medium)
  • Used in cell culture: The human mammary epithelial cell MCF-10A cells were maintained in DMEM/F12 and supplemented with hydrocortisone along with other components
  • The SUM149 cell line was established from a primary IBC and grown in FBS supplemented with Ham′s F12 medium containing insulin and hydrocortisone
  • Used in differentiation assays of melanoma spheroid cells

Biochem/physiol Actions

Primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na2+ retention potency.

Physical form

powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw

Reconstitution

To prepare 50 μg/mL stock solution; add 1 mL absolute ethanol, gently swirl to dissolve, add 19 mL sterile medium while mixing.

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Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Repr. 1A - STOT RE 2

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Hydrocortisone United States Pharmacopeia (USP) Reference Standard

USP

1316004

Hydrocortisone

Hydrocortisone British Pharmacopoeia (BP) Assay Standard

BP576

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