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H3506

Sigma-Aldrich

Hyaluronidase from bovine testes

Type I-S, lyophilized powder, 400-1000 units/mg solid

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Synonym(s):
Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

type

Type I-S

Quality Level

form

lyophilized powder

specific activity

400-1000 units/mg solid

mol wt

~55 kDa (four subunits of 14 kDa each)

technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

UniProt accession no.

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

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vibrant-m

H3506

Hyaluronidase from bovine testes

vibrant-m

H3884

Hyaluronidase from bovine testes

vibrant-m

H3757

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vibrant-m

H6254

Hyaluronidase from sheep testes

specific activity

400-1000 units/mg solid

specific activity

750-3000 units/mg solid

specific activity

300-1,000 U/mg

specific activity

≥1,500 units/mg solid

form

lyophilized powder

form

lyophilized powder (essentially salt-free)

form

lyophilized powder

form

lyophilized powder

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing

mol wt

~55 kDa (four subunits of 14 kDa each)

mol wt

~55 kDa (four subunits of 14 kDa each)

mol wt

~55 kDa (four subunits of 14 kDa each)

mol wt

55 kDa

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

Hyaluronidase from bovine testes has been used for the digestion of breast tissue, heart tissue and oocytes for isolation of cells.

Suitable for use in preparation of single cell suspension for sequencing.

Biochem/physiol Actions

Hyaluronidase degrades hyaluronan and has been found to be inappropriately regulated during cancer progression.
These enzymes randomly cleave β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

recommended

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Heikki J Nieminen et al.
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A SAGE (serial analysis of gene expression) view of breast tumor progression.
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Cancer Research, 61, 5697-5697 (2001)
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The American journal of pathology, 176(3), 1421-1432 (2010-01-30)
Mutation and loss of function in p53 are common features among human breast cancers. Here we use BALB/c-Trp53+/- mice as a model to examine the sequence of events leading to mammary tumors. Mammary gland proliferation rates were similar in both
Effect of pressure at primary drying of freeze-drying mouse sperm reproduction ability and preservation potential.
Kawase Y et al.
Reproduction (Cambridge, England), 133, 841-841 (2007)

Articles

Use of MULTI-seq lipid-modified oligos, protocol, and troubleshooting guide for PCR Assays and Sequencing applications.

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Protocols

This procedure may be used for Hyaluronidase products.

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