H3663

Anti-HA antibody, Mouse monoclonal

clone HA-7, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin
• UNSPSC Code: 12352203
• NACRES: NA.56
• MDL number: MFCD00803873
• Conjugate: unconjugated
• Clone: HA-7, monoclonal
• Application: ICC, IP, WB
• Technique(s): immunocytochemistry: 1.0-2.0 μg/mL, immunoprecipitation (IP): 0.5-2.0 μg, western blot: 0.25-1 μg/mL
• Citations: 954

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: HA-7, monoclonal
• concentration: ~1 mg/mL
• technique(s): immunocytochemistry: 1.0-2.0 μg/mL, immunoprecipitation (IP): 0.5-2.0 μg, western blot: 0.25-1 μg/mL
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• Quality Level: 200

Description

Application

Monoclonal Anti-HA antibody produced in mouse has been used:

• in immunoblotting[1]
• in immunocytochemistry[2]
• in the immunoprecipitation[3]

Biochem/physiol Actions

Hemagglutinin (HA) binds to acid binding and is essential for the initiation of virus infection.[4] It is the key target for the generation of vaccine.[5] HA is the dominant antigen against which humoral immune response is provoked. Vaccines development involves the generation of antibody specific to the variable head and the conserved stalk region. Both the anti-stalk and anti-head antibodies may elicit protective function in influenza disease.[6]

The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.

General description

Hemagglutinin (HA) is a glycoprotein present in the membrane of influenza virus. HA exists as a trimer and each monomer at its membrane tip has receptor-binding site.[4] Glycosylation event of HA occurs in endoplasmic reticulum.[5] The head region with the receptor-binding site of the HA is variable. The mouse monoclonal Anti-HA, Clone HA-7 recognizes the human influenza virus hemagglutinin (HA), known as the HA tag. The product is reactive with HA-tagged fusion proteins expressed at either the amino or the carboxy terminus of the fusion protein.

Immunogen

synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide as a preservative.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)