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H8032

Sigma-Aldrich

Hanahan′s Broth (SOB Medium)

powder microbial growth medium

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Synonym(s):
Super Optimal Broth

grade

for molecular biology

Quality Level

description

recommended for cultivation of recombinant E. coli.

sterility

non-sterile

form

powder

composition

KCl, 0.186 g/L
MgSO4, 2.4 g/L
NaCl, 0.5 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

pH

6.8-7.2(2.8% solution)

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

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Hanahan′s Broth (SOB Medium)

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suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

-

technique(s)

microbiological culture: suitable

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

sterility

non-sterile

composition

KCl, 0.186 g/L , NaCl, 0.5 g/L , Yeast extract, 5 g/L , MgSO4, 2.4 g/L , Tryptone, 20 g/L

composition

Agar, 15 g/L , NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

composition

NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

composition

NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L

application(s)

food and beverages
microbiology

application(s)

agriculture
food and beverages
microbiology

application(s)

food and beverages

application(s)

food and beverages
microbiology

General description

Hannahan’s Broth (SOB) is a microbial growth medium used for the preparation and transformation of competent cells (E. coli). Casein enzymic hydrolysate and yeast extract supply nitrogenous compounds and growth factors for recombinant Escherichia coli. Potassium chloride and sodium chloride maintain the isotonic condition. Magnesium sulfate is added to the medium as a necessary component for DNA replication.

Application

Hanahan′s Broth is recommended for use in cultivation of recombinant strains of Escherichia coli. It is suitable for the preparation of competent E. coli., often with the addition of 0.4% dextrose. It has also been used for the preparation of C. necator H16 competent cells. It is suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.

Features and Benefits

Hannahan′s Broth (SOB) powder provides:
  • Standard formulation
  • An inexpensive alternative to liquid

Reconstitution

Stir to suspend 28 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Other Notes

Casein enzymic hydrolysate and yeast extract supply the necessary nitrogenous compounds and growth factors. Potassium chloride and sodium chloride provide an isotonic environment for the cells. Magnesium sulfate aids DNA replication.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Exploiting biological processes to recycle renewable carbon into high value platform chemicals provides a sustainable and greener alternative to current reliance on petrochemicals. In this regard Cupriavidus necator H16 represents a particularly promising microbial chassis due to its ability to
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Arenas-LC, et al.
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Molecular Cloning: A Laboratory Manual, 1-1 (1989)
D Hanahan
Journal of molecular biology, 166(4), 557-580 (1983-06-05)
Factors that affect the probability of genetic transformation of Escherichia coli by plasmids have been evaluated. A set of conditions is described under which about one in every 400 plasmid molecules produces a transformed cell. These conditions include cell growth

Protocols

General protocols for growth of competent cells in microbial medium.

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