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Hanks′ Balanced Salt solution

Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture


Quality Level






cell culture | mammalian: suitable


endotoxin, tested


NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: no

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General description

Hanks’ Balanced Salt solution (HBSS) is a buffer that mimics biological medium and stabilizes cells. It is useful in leucocyte labeling studies. HBSS is an effective preservative for periodontal ligament cells. HBSS lacks essential inorganic ions and is useful in starvation studies.


Hanks′ Balanced Salt solution has been used:
  • as a rinse solution for hepatocytes
  • for hippocampi dissection
  • in corrosion testing of dog-bone specimens
  • for starvation studies in breast cancer cell line
  • as a component of dissociation solution for dorsal root ganglion
  • as a chamber solution for the patch-clamp apparatus

Other Notes

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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Physical removal of the midbody remnant from polarised epithelial cells using take-up by suction pressure (TUSP)
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Emmet Galvin et al.
Medical & biological engineering & computing, 55(8), 1261-1275 (2016-10-28)
Magnesium stents are a promising candidate in the emerging field of absorbable metallic stents (AMSs). In this study, the mechanical and corrosion performance of dog-bone specimens and a specific stent design of a magnesium alloy, WE43, are assessed experimentally in
Online oxygen monitoring using integrated inkjet-printed sensors in a liver-on-a-chip system
Moya A, et al.
Lab on a chip, 18, 2023-2035 (2018)
Hank s balanced salt solution: an alternative resuspension medium to label autologous leukocytes. Experience in inflammatory bowel disease
Martin-Comin J, et al.
Brazilian Archives of Biology and Technology, 45, 39-44 (2002)
Vladimir Benes et al.
Scientific reports, 5, 11590-11590 (2015-06-26)
microRNAs are an abundant class of small non-coding RNAs that control gene expression post-transcriptionally. Importantly, microRNA activity participates in the regulation of cellular processes and is a potentially valuable source of biomarkers in the diagnosis and prognosis of human diseases.


Detection of Endogenous Pathway Activity in Novel Reporter Cell Lines

Our company presents an article on the Detection of Endogenous Pathway Activity in Novel Reporter Cell Lines

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