H9914

HIS-Select^® Nickel Magnetic Agarose Beads

• Synonym(s): nickel charged magnetic beaded agarose
• NACRES: NA.56

Properties

• conjugate: magnetic beads
• Quality Level: 200
• form: suspension
• shelf life: 2 yr (Unopened product)
• matrix: 6% beaded magnetic agarose
• capacity: ≥15 mg/mL binding capacity
• storage temp.: 2-8°C

Description

General description

HIS-Select Magnetic Agarose Beads consist of paramagnetic, immobilized metal-ion affinity chromatography (IMAC) resin that contain a proprietary quadridentate chelate, which is bound with nickel and covalently attached through a non-charged, hydrophilic linker to magnetic beaded agarose. The magnetic properties of the beads aid in manipulations, such as repetitive washings, and recovery of the protein bound beads. This leads to greater experimental reproducibility and more accurate quantitation of the His-tagged proteins of interest.

Packaging

1, 5 mL

Application

The HIS-Select Nickel Magnetic Agarose Beads are designed for use in automated and small-scale affinity capture (molecular pull-down) purifications of histidine-tagged protein / His-tag protein while exhibiting low non-specific binding of other proteins. The beads can be used to purify His-tagged proteins under native and denaturing conditions.

Physical form

Supplied as a 50% slurry suspension in 30% Ethanol.

Legal Information

HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS02,GHS07,GHS08
• Signal Word: Danger
• Hazard Statements: H226 - H317 - H350i - H360D - H373 - H412
• Precautionary Statements: P202 - P210 - P273 - P280 - P303 + P361 + P353 - P308 + P313
• Hazard Classifications: Aquatic Chronic 3 - Carc. 1A Inhalation - Flam. Liq. 3 - Repr. 1B - Skin Sens. 1 - STOT RE 2
• Target Organs: Respiratory Tract
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 86.0 °F
• Flash Point(C): 30 °C