I6784

Monoclonal Anti-Interleukin-2 antibody produced in mouse

clone 5334, purified immunoglobulin, lyophilized powder

• Synonym(s): Anti-IL2, Anti-Lymphokine, Anti-TCGF
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5334, monoclonal
• form: lyophilized powder
• species reactivity: human
• technique(s): flow cytometry: 25 μg/mL; immunohistochemistry: 8-25 μg/mL; neutralization: suitable
• isotype: IgG1
• UniProt accession no.: P60568
• storage temp.: −20°C
• Gene Information: human ... IL2(3558)

Description

General description

Interleukin-2 (IL-2) is a cytokine produced by activated T cells in response to antigen stimulation. IL-2 is important for proliferation of lymphocytes, natural killer cells and macrophages and in the differentiation of CD4⁺ cells into Th1 and Th2 effector cells. The effects of IL-2 are mediated by the signalling activated by its binding to IL-2 receptor (IL-2 R). IL-2 activates key signalling pathways such as MAPK, JAK/STAT as well as PI3K/Akt. Activation of these pathways modulates production of inflammatory cytokines, survival and progression through cell cycle
Monoclonal Anti-Interleukin-2 recognizes human Interleukin-2. It does not react with mouse IL-2.

Immunogen

purified, E. coli-derived recombinant human interleukin-2.

Application

Anti-interleukin-2 antibody may be used in immunohistochemistry at a working concentration of 8-25 μg/ml in human peripheral blood leukocytes. For flow cytometry a concentration of 25 μg/ml may be used. The antibody is suitable for IL-2 neutralization assays.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves