I9031

Monoclonal Anti-Intracellular Adhesion Molecule 3 antibody produced in mouse

~1 mg/mL, clone 186-2G9, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-ICAM-3
• MDL number: MFCD01094485
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 186-2G9, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 120 kDa
• species reactivity: human
• concentration: ~1 mg/mL
• technique(s): direct immunofluorescence: 2 μg using 1 mg protein lysate; flow cytometry: 2 μg using 1 mg protein lysate; immunohistochemistry: 2-4 μg/mL using human tonsil tissue sections; immunoprecipitation (IP): 2 μg using 1 mg protein lysate; western blot: 1-5 μg/mL
• UniProt accession no.: P32942
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... ICAM3(3385)

Description

General description

The gene ICAM3 (intercellular adhesion molecule 3) encodes type I membrane glycoprotein that belongs to the immunoglobulin (ig) superfamily. The encoded protein is extensively glycosylated and contains five Ig-like domains that share high homology with corresponding domains in ICAM-1 and ICAM-2. ICAM3 is found to be expressed on all leukocytes, including the resting leukocytes. The gene is mapped to human chromosome 19p13.2.

Immunogen

stimulated human leukocytes.

Biochem/physiol Actions

The protein intercellular adhesion molecule 3 (ICAM-3) functions as a receptor for LFA-1 (leukocyte function-associated antigen-1) and mediates adhesive interactions of leukocyte cell surface adhesion molecules in inflammatory and immune response. LFA-1 participates in several processes, such as T cell-mediated killing, T helper and B lymphocyte responses, natural killing, Ab-dependent cellular cytotoxicity by monocytes and granulocytes, and adherence of leukocytes to endothelial cells, fibroblasts, and epithelial cells. ICAM-3 mediates the adhesion of resting T lymphocytes to LFA-1 and participates in the initiation of immune responses. It may also function in lymphocyte activation, as well as in adhesion.

Physical form

Solution in phosphate buffered saline pH 7.4, containing 0.08% sodium azide.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable