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L5542

Sigma-Aldrich

LB Agar Plates

pre-poured LB agar plates for E. coli

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Synonym(s):
pre-poured LB agar plates

grade

for molecular biology

sterility

sterile; autoclaved

form

plates (10 cm)
plates (10cm, 20-25ml each)

capacity

20-25 mL

storage temp.

2-8°C

suitability

nonselective for Escherichia coli
nonselective for coliforms

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of Escherichia coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins.
LB Agar Plates has been used:
  • to culture bacteria like, Escherichia coli strain INV- α and methicillin-susceptible Staphylococcus aureus
  • to culture numerous bacteria and is used in strain storage method
  • to analyse the egestion of midgut microbes during sand fly bites

Features and Benefits

Lennox LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation

Components

15g/L Agar
10g/L Tryptone
5 g/L Yeast Extract
5 g/L NaCl

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ
Kuru E, et al.
Nature Protocols, 10(1), 33-33 (2015)
Casper Hyttel Clausen et al.
Sensors (Basel, Switzerland), 18(10) (2018-10-20)
Monitoring of bacteria concentrations is of great importance in drinking water management. Continuous real-time monitoring enables better microbiological control of the water and helps prevent contaminated water from reaching the households. We have developed a microfluidic sensor with the potential
Bacteria Detection and Differentiation Using Impedance Flow Cytometry
Clausen C, et al.
Sensors, 18(10), 3496-3496 (2018)
Priyanka Karicherla et al.
Investigative ophthalmology & visual science, 50(1), 256-262 (2008-09-09)
Nona-D-arginine amide (D9R) was evaluated as treatment for Pseudomonas aeruginosa keratitis when administered alone and with ciprofloxacin. Mouse corneas were infected with P. aeruginosa. Immediately after infection and hourly for 5 hours, eyes received 5 microL of either Dulbecco phosphate-buffered
Gut microbes egested during bites of infected sand flies augment severity of leishmaniasis via inflammasome-derived IL-1beta
Dey R, et al.
Cell host & microbe, 23(1), 134-143 (2018)

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