Merck
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M5815

Sigma-Aldrich

Monoclonal Anti-Myogenin antibody produced in mouse

clone F12B, purified immunoglobulin, buffered aqueous solution

Synonym(s):
Anti-MYF4, Anti-bHLHc3, Anti-myf-4
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

F12B, monoclonal

form

buffered aqueous solution

mol wt

antigen 34 kDa

species reactivity

mouse, rat, human

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using rhabdomyosarcoma tissue sections
immunoprecipitation (IP): 2 μg using 1 mg protein lysate
indirect ELISA: suitable
indirect immunofluorescence: suitable
western blot: 1 μg/mL

isotype

IgG1

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... MYOG(4656)
mouse ... Myog(17928)
rat ... Myog(29148)

General description

Myogenin belongs to the basic helix-loop-helix (bHLH) superfamily.
Myogenin is a transcription factor present in the nucleus. It possesses an amino-acid rich region and a helix-loop-helix structure. The gene encoding it is localized on human chromosome 1q31-41 and has three exons.

Immunogen

rat recombinant myogenin (amino acids 30-224).

Application

Monoclonal Anti-Myogenin antibody produced in mouse has been used in immunohistochemistry, immunofluorescence and western blotting.

Biochem/physiol Actions

Myogenin acts as a biomarker in rhabdomyosarcoma (RMS). It has a role in developmental processes, such as hematopoiesis, neurogenesis, myogenesis, tumor cell invasion and metastasis.
Myogenin acts as a regulator during myoblast and skeletal muscle differentiation. It is considered as a definite marker for skeletal muscle differentiation.

Physical form

Solution in phosphate buffered saline containing 0.08% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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SDS

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