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M6895

Sigma-Aldrich

MEM Vitamin Solution (100×)

sterile-filtered, BioReagent, suitable for cell culture

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eCl@ss:
32160406
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

200

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

yellow to dark yellow

pH

7.0-8.0

application(s)

agriculture

shipped in

dry ice

storage temp.

−20°C

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MEM Vitamin Solution (100×)

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MEM Non-essential Amino Acid Solution (100×)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

color

yellow to dark yellow

color

-

color

yellow to dark yellow

color

-

application(s)

agriculture

application(s)

-

application(s)

-

application(s)

-

shipped in

dry ice

shipped in

ambient

shipped in

dry ice

shipped in

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

General description

MEM Vitamin Solution (100×) is a sterile-filtered bioreagent, which is suitable for cell culture.
Synthetic/ basal media used currently contain amino acids, carbohydrates, vitamins and salts. Eagle′s minimum essential medium (MEM) is a modified version of BME (basal medium Eagle′s), which contains increased amounts of each amino acid. Addition of water soluble vitamins such as, inositol to various culture facilitates the hatching of hamster and rabbit blastocysts. Vitamins also influence the metabolism of glucose in sheep and mouse embryos.

Application

MEM Vitamin Solution (100×) has been used in:
  • glial conditioned medium for primary neuronal culture
  • in Luria-Bertani (LB) medium or minimal medium (MM)
  • in private medium (PM = aminoacid-starved medium) for the Aa deprivation experiments

MEM Vitamin Solution (100×) is used for the following applications:
  • In the preparation of DMEM medium (for primary neural cell culture)
  • For the maintenance of cell lines in Eagle′s Minimum Essential Medium (the medium is supplemented with MEM Vitamin Solution along with other components)

Adding supplements of vitamins to media stimulates growth and prolongs the viability of cells in culture. This concentrated supplement adds nutrients and reduces the biosynthetic burden on cell in vitro.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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