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Anti-FLAG® M2 Magnetic Beads

affinity isolated antibody

Monoclonal ANTI-FLAG® M2 antibody produced in mouse, FLAG® magnetic affinity resin, FLAG® resin for high throughput, Flag® Affinity resin, Anti-ddddk, Anti-dykddddk


magnetic beads

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies


M2, monoclonal



shelf life

2 yr at -20 °C

analyte chemical class(es)

proteins (FLAG® Affinity Gels, FLAG® tag, 3x FLAG® tag, DYKDDDDK tag)


affinity chromatography: suitable
immunoprecipitation (IP): suitable

bead size

20-75 μm


superparamagnetic iron impregnated 4% agarose bead, with an average diameter of 50 μm.




≥0.6 mg/mL binding capacity

shipped in

wet ice

storage temp.


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General description

Anti-FLAG M2 Magnetic Beads are 4% agarose beads bound with the Anti-FLAG M2 (mouse monoclonal) antibody. The M2 antibody recognizes the FLAG sequence at the N-terminus, Met-N-terminus and C-terminus. This alows for detection and capture of fusion proteins containing a FLAG peptide sequence.


Suitable for immunoprecipitation procedures.

Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide

Browse additional application references in our FLAG® Literature portal.


1, 5 mL

Features and Benefits

The magnetic properties allow for:
- Very rapid separation
- Significantly accelerated manipulations, such as repetitive washings
- Processing of multiple samples performed in plate formats
This leads to:
- Faster experimentation
- Better reproducibility
- More accurate quantitation of the proteins of interest

Physical form

Supplied as a 50% suspension in 50% glycerol with 10mM sodium phosphate, 150mM sodium chloride, pH 7.4 and 0.02% (w/v) sodium azide (PBA/A).

Other Notes

Do not use magnetic stirring system as this will destroy the resin beads.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

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