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M9170

Sigma-Aldrich

Macrophage Colony-Stimulating Factor from mouse

M-CSF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

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Synonym(s):
mCSF-1, mM-CSF, CSF-1, M-CSF
CAS Number:
EC Number:
MDL number:
eCl@ss:
32190102

biological source

mouse

Quality Level

recombinant

expressed in E. coli

Assay

≥98% (SDS-PAGE)

form

lyophilized powder

potency

0.500-3.000 ng/mL ED50

quality

endotoxin tested

mol wt

dimer 18.2 kDa (containing 156 amino acid residues)

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1.000 EU/μg

color

white

UniProt accession no.

storage temp.

−20°C

Gene Information

mouse ... Csf1(12977)

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This Item
SRP3050SRP3247G8160
Sigma-Aldrich

Sigma-Aldrich

SRP3247

M-CSF from rat

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

assay

≥98% (SDS-PAGE)

assay

≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

>97% (SDS-PAGE)

form

lyophilized powder

form

lyophilized

form

lyophilized

form

lyophilized powder

potency

0.500-3.000 ng/mL ED50

potency

≤0.1 ng/mL

potency

≤5.0 ng/mL ED50

potency

10-60 pg/mL ED50

mol wt

dimer 18.2 kDa (containing 156 amino acid residues)

mol wt

14.6 kDa

mol wt

18.1 kDa

mol wt

19 kDa (179 amino acids including N-terminal methionine)

Application

Macrophage Colony-Stimulating Factor from mouse has been used:
  • in the initiation of osteoclastogenesis in bone marrow monocyte cells
  • to stimulate osteoclast differentiation in non-adherent hematopoietic cell
  • for the generation of blood-derived stem cells

Biochem/physiol Actions

Macrophage Colony Stimulating Factor (M-CSF), also known as CSF-1, can be produced by a number of cells, including fibroblasts, monocytes, activated macrophages, secretory epithelial cells of the endometrium, endothelial cells activated by LPS or cytokines, and bone marrow stromal cells. In addition to its namesake activity, M-CSF can stimulate the proliferation of isolated macrophages, augment the production and release of cytokines and other inflammatory modulators from macrophages, enhance macrophage antibody-dependent cell-mediated cytotoxicity, prime and enhance macrophages in their ability to kill tumor cells and other microorganisms, stimulate pinocytosis, and support osteoclast differentiation. M-CSF binds and activates a 165 kDa glycoprotein of the receptor tyrosine kinase subclass III or the RTK subfamily. Due to alternative splicing of a single M-CSF gene and due to variations in glycosylation, the molecular weight of natural soluble M-CSF ranges from 44-86 kDa. Human and mouse M-CSF share approximately 80% sequence homology in the conserved N-terminal region of 150 amino acids. Although human M-CSF is active in murine systems, mouse M-CSF appears to be species-specific in its actions.

Physical form

Lyophilized from a sterile filtered buffered aqueous solution.

Analysis Note

The proliferative activity is tested by the dose-dependent stimulation of the murine monocytic cell line, M-NFS-60.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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