Merck
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MAK193

Sigma-Aldrich

Citrate Synthase Activity Assay Kit

Sufficient for 100 Colorimetric tests

EC Number:
NACRES:
NA.84

detection method

colorimetric

storage temp.

−20°C

Gene Information

human ... CS(1431)
mouse ... CS(12974)
rat ... CS(170587)

Related Categories

General description

Citrate Synthase (CS; EC 2.3.3.1) is a mitochondrial enzyme found in all cells capable of oxidative metabolism. It catalyzes the synthesis of citrate from oxaloacetate in the Krebs tricarboxylic acid cycle.1,2 CS is involved in energy production, lipogenesis, and cholesterologenesis, and its activity follows a circadian pattern.

Application

Citrate Synthase Activity Assay Kit has been used to measure the activity of the enzyme citrate synthase in samples.

Suitability

Suitable for the detection of citrate synthase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Principle

The Citrate Synthase Activity Assay kit provides a simple and direct procedure for measuring the activity of CS in a variety of tissues, cell cultures (adherent or suspension), and purified mitochondria. CS activity is determined using a coupled enzyme reaction, which results in a colorimetric (412 nm) product proportional to the enzymatic activity present. One unit of CS is the amount of enzyme that generates 1.0 mmole CoA per minute at 25 °C and pH 7.2.

Kit Components Only

Product No.
Description

  • CS Assay Buffer

  • CS Substrate Mix

  • CS Developer

  • GSH Standard, Reduced

  • CS Positive Control

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

Certificate of Analysis

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Certificate of Origin

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