Merck
All Photos(3)

Documents

MBD6000

Sigma-Aldrich

5R-Plex kit

Ultra-sensitive 16S NGS assay for degraded and low biomass DNA

Sign Into View Organizational & Contract Pricing

Select a Size

Change View

Select a Size

Change View

Quality Level

usage

Preparing 96 samples for sequencing

technique(s)

DNA amplification: suitable
DNA sequencing: suitable

shipped in

dry ice

storage temp.

-10 to -25°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SEQXEMBD0027SEQXI
5R-Plex kit Ultra-sensitive 16S NGS assay for degraded and low biomass DNA

Sigma-Aldrich

MBD6000

5R-Plex kit

SEQPLEX-I WGA Kit Whole Genome Amplification, DNA Amplification

Sigma-Aldrich

SEQXI

SEQPLEX-I WGA Kit

usage

Preparing 96 samples for sequencing

usage

-

usage

-

usage

-

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

storage temp.

-10 to -25°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

General description

The 16S ribosomal RNA gene (16S rRNA) is a common bacterial marker, used as a target for microbial community profiling in microbiome metagenomic studies. The gene is composed of nine variable regions (V1-V9) interspersed between conserved regions. The most common 16S rRNA NGS assays target one or two regions using a single set of primers (e.g., V3-V4). However, this approach usually results in a poor bacterial detection and classification rate when the DNA input is of low-quality or when applying it on extremely low biomass samples.  The 5R-PLEX kit offers a complete solution to challenging biological samples where the standard 16S sequencing protocols fail to perform.   Sample types include:
  • Formalin-Fixed, Paraffin-Embedded (FFPE) tissue
  • Cancerous tumor tissue 
  • Degraded or damaged DNA  
  • Low biomass samples 
  • Fossil-derived and ancient DNA
The 5R-PLEX kit was developed in collaboration with Dr. Ravid Straussman of the Weissmann Institute, based off a technique that was featured in his Science publication focused on the human tumor microbiome characterization (Science2020 May 29;368(6494):973-980. doi: 10.1126/science.aay9189) . The kit targets five short variable regions along the 16S rRNA gene (V2, V3, V5, V6, V8) that are co-amplified in a multiplexed PCR within a single tube.   After sequencing, the output data is uploaded to our M-CAMPTM platform (Microbiome Computational Analysis for Multi-omics Profiling) and analyzed using the unique 5R-PLEX algorithm[2]. This new analysis approach computationally combines the amplified regions to reconstruct one coherent high-resolution microbial profile. Read more about our kit here.

Features and Benefits

  • The 5R-PLEX Kit provides high profile sequencing from degraded, damaged or low biomass samples
  • Multiplexed NGS assay targets 5 variable regions of the 16S rRNA gene in a single primer pool
  • Includes positive control for trouble shooting experiments.
  • The kit can detect as low as 1pg of highly degraded bacterial DNA with an average fragment size of 260bp.
  • Kit includes low bioburden reagents
  • Includes exclusive access to M-CAMP platform featuring an innovative algorithm designed to reconstruct a single coherent microbial profiling and perform comprehensive analysis.

Components

  • 5R-PLEX PCR1 Primer mix- 25 μL
  • 5R-PLEX PCR2 Forward Primer mix- 25 μL
  • 5R-PLEX index plate 96-plate
  • Water, microbial DNA-free 10X- 1.5 mL
  • HF DNA Polymerase- 0.1 mL
  • 5X HF buffer 2 mL
  • dNTP′s- 0.2 mL
  • Elution Buffer (EB), microbial DNA-free- 8 mL
  • 5R-PLEX Positive Control (10 ng/μL)- 30 μL

Storage and Stability

The shelf life of all reagents provided in the kit is 12 months when stored properly. Store all components at -20 °C.

Other Notes

Kit component details can be found in the User guide.

Legal Information

M-Camp is a trademark of Merck KGaA, Darmstadt, Germany

Hazard Statements

Precautionary Statements

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Deborah Nejman et al.
Science (New York, N.Y.), 368(6494), 973-980 (2020-05-30)
Bacteria were first detected in human tumors more than 100 years ago, but the characterization of the tumor microbiome has remained challenging because of its low biomass. We undertook a comprehensive analysis of the tumor microbiome, studying 1526 tumors and
Nicole M Davis et al.
Microbiome, 6(1), 226-226 (2018-12-19)
The accuracy of microbial community surveys based on marker-gene and metagenomic sequencing (MGS) suffers from the presence of contaminants-DNA sequences not truly present in the sample. Contaminants come from various sources, including reagents. Appropriate laboratory practices can reduce contamination, but
Amnon Amir et al.
Nucleic acids research, 41(22), e205-e205 (2013-11-12)
The emergence of massively parallel sequencing technology has revolutionized microbial profiling, allowing the unprecedented comparison of microbial diversity across time and space in a wide range of host-associated and environmental ecosystems. Although the high-throughput nature of such methods enables the
Jacob T Nearing et al.
Microbiome, 9(1), 113-113 (2021-05-20)
Advances in DNA sequencing technology have vastly improved the ability of researchers to explore the microbial inhabitants of the human body. Unfortunately, while these studies have uncovered the importance of these microbial communities to our health, they often do not
Garold Fuks et al.
Microbiome, 6(1), 17-17 (2018-01-28)
Most of our knowledge about the remarkable microbial diversity on Earth comes from sequencing the 16S rRNA gene. The use of next-generation sequencing methods has increased sample number and sequencing depth, but the read length of the most widely used

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service