N1408

NGF-β human

from human, recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

• Synonym(s): HSAN5, NGFB, hNGFβ
• MDL number: MFCD00164982
• NACRES: NA.77

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in NSO cells
• Assay: >97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.15-3.0 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• packaging: pkg of 5X0.1 mg; pkg of 0.1 mg
• technique(s): cell culture | mammalian: suitable
• impurities: <1 EU/μg
• solubility: water: soluble 0.1 g/L at 20 °C
• UniProt accession no.: P01138
• storage temp.: −20°C
• Gene Information: human ... NGF(4803)

Description

General description

The nerve growth factor β (NGFB) gene is mapped to human chromosome 1p13.2.

Application

NGF-β human has been used:

• in protein pin arrays
• to induce U87-MG (glioblastoma cell line) cell proliferation
• in drug release kinetics

Biochem/physiol Actions

Nerve growth factor β (NGFB) acts as a key mediator in neuronal and non-neuronal development, differentiation, and survival. Polymorphism of NGFB gene leads to hereditary sensory and autonomic neuropathies. Charcot arthropathy and thermal insensitivity are few of the related symptoms. NGFβ is known to promote cancer cell proliferation and metastasis, also mediates angiogenesis. It facilitates the development of various cancer including, oral, breast, lungs, pancreatic and prostate cancers. Elevated expression of NGFβ contributes to progression of human cholangiocarcinoma. In animal models, NGFβ is observed to prevent cholinergic neuron loss and enhances memory. NGF-β promotes the survival and cholinergic phenotype of basal forebrain cholinergic neurons and stimulates neurite outgrowth of embryonic trigeminal motor neurons in vitro.

Physical form

Lyophilized from 0.2% acetic acid containing 5 mg bovine serum albumin.

Analysis Note

The biological activity is measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves