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Oil Red O solution

0.5% in isopropanol

Sudan Red 5B, Solvent Red 27
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
EC Number:



Quality Level


0.5% in isopropanol


0.20-0.50 at 510-517 nm


diagnostic assay manufacturing

storage temp.

room temp



InChI key


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General description

Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.


Oil red O solution has been used to stain neutral lipids and adipocytes and to visualize fat droplets in fibroblasts.
Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.


500 mL in glass bottle


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Signal Word


Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids



Flash Point(F)

53.6 °F - closed cup

Flash Point(C)

12.0 °C - closed cup

Certificate of Analysis

Certificate of Origin

Hepatic Peroxisome Proliferator-Activated Receptor Gamma Signaling Contributes to Alcohol-Induced Hepatic Steatosis and Inflammation in Mice.
Zhang W, et al.
Alcoholism, Clinical and Experimental Research, 40(5), 988-999 (2016)
LWW Doody's all reviewed collection, McClatchey: Clinical Laboratory Medicine
Clinical Laboratory Medicine (2002)
Directly reprogramming fibroblasts into adipogenic, neurogenic and hepatogenic differentiation lineages by defined factors.
Wu W, et al.
Experimental and Therapeutic Medicine, 13(6), 2685-2690 (2017)
Sulforaphane inhibits mammary adipogenesis by targeting adipose mesenchymal stem cells.
Li Q, et al.
Breast Cancer Research and Treatment, 141(2), 317-324 (2013)
Pablo Sanchez Bosch et al.
Developmental cell, 51(6), 787-803 (2019-11-19)
The use of adult Drosophila melanogaster as a model for hematopoiesis or organismal immunity has been debated. Addressing this question, we identify an extensive reservoir of blood cells (hemocytes) at the respiratory epithelia (tracheal air sacs) of the thorax and


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