All Photos(1)

O1391

Sigma-Aldrich

Oil Red O solution

0.5% in isopropanol

Synonym(s):
Sudan Red 5B, Solvent Red 27
Empirical Formula (Hill Notation):
C26H24N4O
CAS Number:
Molecular Weight:
408.49
EC Number:
NACRES:
NA.47

form

liquid

Quality Level

concentration

0.5% in isopropanol

εmax

0.20-0.50 at 510-517 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C26H24N4O/c1-16-9-10-17(2)22(13-16)27-28-23-14-19(4)24(15-18(23)3)29-30-26-21-8-6-5-7-20(21)11-12-25(26)31/h5-15,29H,1-4H3/b28-27?,30-26-

InChI key

HJAYEODIXUYVIC-LTFAAIOASA-N

Looking for similar products? Visit Product Comparison Guide

General description

Oil Red O stains lipid materials and imparts red-orange color to the lipids. It also marks vacuoles, particularly observed in ALL-L3 (acute lymphoblastic leukemia) or Burkitt′s lymphoma/leukemia. Oil Red O staining is done on fresh samples, as alcohol fixation removes lipids.

Application

Oil red O solution has been used to stain neutral lipids and adipocytes and to visualize fat droplets in fibroblasts.
Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.

Packaging

500 mL in glass bottle

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

53.6 °F - closed cup

Flash Point(C)

12.0 °C - closed cup

Certificate of Analysis

Certificate of Origin

Hepatic Peroxisome Proliferator-Activated Receptor Gamma Signaling Contributes to Alcohol-Induced Hepatic Steatosis and Inflammation in Mice.
Zhang W, et al.
Alcoholism, Clinical and Experimental Research, 40(5), 988-999 (2016)
LWW Doody's all reviewed collection, McClatchey: Clinical Laboratory Medicine
Clinical Laboratory Medicine (2002)
Directly reprogramming fibroblasts into adipogenic, neurogenic and hepatogenic differentiation lineages by defined factors.
Wu W, et al.
Experimental and Therapeutic Medicine, 13(6), 2685-2690 (2017)
Sulforaphane inhibits mammary adipogenesis by targeting adipose mesenchymal stem cells.
Li Q, et al.
Breast Cancer Research and Treatment, 141(2), 317-324 (2013)
Pablo Sanchez Bosch et al.
Developmental cell, 51(6), 787-803 (2019-11-19)
The use of adult Drosophila melanogaster as a model for hematopoiesis or organismal immunity has been debated. Addressing this question, we identify an extensive reservoir of blood cells (hemocytes) at the respiratory epithelia (tracheal air sacs) of the thorax and

Articles

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

Mesenchymal Stem Cell FAQs

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Protocols

Trilineage Differentiation of Multipotent Human Mesenchymal Stem Cells (MSCs) into Osteocytes, Adipocytes and Chondrocytes

Osteogenesis, adipogenesis and chondrogenesis differentiation protocols and media for human mesenchymal stem cell culture. Explore over 350 PromoCell products on our company website.

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service