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O4387

Sigma-Aldrich

Oligo(dT)23, Anchored

70 μM in H2O

NACRES:
NA.52

usage

0.1 mL sufficient for 100 RT-PCR reactions (as described in the Technical Bulletin for Product Codes HSRT100 and HSRT20)

Quality Level

concentration

70 μM in H2O

color

colorless

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

Oligo(dT)23, Anchored primers are used to prime mRNA with a poly(A) tail for cDNA synthesis. The primers have 23 thymidine residues and one G, C, or A residue (the anchor) at the 3′ end. This anchor ensures that the oligo(dT) primer binds at the very beginning of the message and there is not a long region of the unusable sequence.

Application

Oligo(dT)23, Anchored has been used in the synthesis of cDNA for real-time quantitative polymerase chain reaction (PCR).
The Anchored Oligo(dT)23 Primers have 23 thymidine residues and one G, C, or A residue (the anchor) at the 3′ end. This anchor ensures the oligo(dT)23 primer binds at the beginning of the message such that there are no long regions of unusable sequence. Anchored oligo(dT)23 primers may provide an advantage over standard oligo(dT) primers when generating cDNA from poly(A)+ RNA.

Packaging

0.1 mL in poly bottle

Features and Benefits

  • Oligo(dT)23, Anchored primers can be used in conjunction with random nonamers (Product No. R 7647) when preparing cDNA libraries, when sequence information is incomplete or absent, or other instances where specific primers are not useful
  • Can be used as an alternative to reverse transcription primers for first-strand cDNA synthesis, cDNA library construction, and other applications
  • The anchored oligo(dT)23 primers will not affect PCR after transcription due to their decreased ability to prime at increased temperatures (up to 65 °C)

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Quotes and Ordering

Articles

Reverse Transcription

One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in half life between different transcripts, the temporal patterns of transcription and the lack of correlation between mRNA and protein.

Protocols

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradation is less than that detected by capillary systems but still sufficient to effect qPCR analyses.

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