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P1243

Sigma-Aldrich

pT7-FLAG-2 Expression Vector

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NACRES:
NA.85

recombinant

expressed in E. coli

Quality Level

grade

for molecular biology

shipped in

dry ice

storage temp.

−20°C

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This Item
F3290P9743P7957
FLAG® Peptide lyophilized powder

Millipore

F3290

FLAG® Peptide

Sigma-Aldrich

Sigma-Aldrich

P7957

C-24 Sequencing Primer

grade

for molecular biology

grade

-

grade

-

grade

for molecular biology

shipped in

dry ice

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

pT7-FLAG-2 is a 4.8 kb E . coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAG® fusion protein containing the FLAG epitope (DYKDDDDK). The promoter region of the very strong phage T7 promoter drives transcription of ORFFLAG fusion constructs. This vector requires the use of E. coli cells containing a source of the T7 polymerase, such as BL21(DE3)T1R cells, Catalog Number B2935.

The C-terminal FLAG fusion protein may be detected using Monoclonal ANTI-FLAG?M2, Catalog Number
F3165, and purified using the ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.

Vector Maps and Sequences

Components

  • pT7-FLAG-2 Expression Vector, 10 μg (P6867) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
  • pT7-FLAG-2-BAP Control Vector 1 μg ( P7117) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
pT7-FLAG is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids


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Retrovirology, 8, 69-69 (2011-08-24)
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Seung-Gi Jin et al.
The Journal of biological chemistry, 280(13), 12700-12709 (2005-02-11)
MBD2 and MBD3 are two proteins that contain methyl-CpG binding domains and have a transcriptional repression function. Both proteins are components of a large CpG-methylated DNA binding complex named MeCP1, which consists of the nucleosome remodeling and histone deacetylase complex
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Communications biology, 4(1), 921-921 (2021-07-31)
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