Each tablet of this product should weigh between 1814.5 and 2005.5 milligrams.
P4417
Phosphate buffered saline
Phosphate buffered saline
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tablet, pH 7.2-7.6 (1 tablet/200 mL)
Synonym(s):
PBS
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50 TABS
MXP 2,131.00
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MXP 3,557.00
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50 TABS
MXP 2,131.00
100 TABS
MXP 3,557.00
About This Item
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Product Name
Phosphate buffered saline, tablet
form
tablet
Quality Level
storage condition
dry at room temperature
color
white
pH
7.2-7.6 (1 tablet/200 mL)
solubility
water: soluble
density
1.02 g/cm3 at 20—25 °C
1.03 g/cm3 at 20—25 °C
application(s)
diagnostic assay manufacturing
histology
life science and biopharma
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General description
Phosphate-buffered saline (PBS) is a widely utilized buffer solution in biological research, valued for its versatility and essential attributes. Notably, its isotonicity closely mirrors the pH, osmolarity, and ion concentrations of the human body, rendering it non-toxic to cells. This pivotal characteristic plays a crucial role in diverse cell biology procedures, including the gentle washing of cells, safe transportation of tissues, and precise dilution of cells and reagents. These applications contribute to the preservation of cell viability and the establishment of a conducive physiological environment. The buffering capacity of PBS, sustained by a phosphate-phosphoric acid equilibrium, establishes a stable pH environment crucial for optimal enzyme activity and various biological processes. This makes PBS an ideal choice for tasks such as preparing reagents, protein dialysis, and immunohistochemistry, ensuring the reproducibility and reliability of experimental results.
Expanding beyond cell biology, PBS demonstrates versatility in applications such as creating custom ELISA buffers for optimal antibody-antigen interactions. Moreover, it serves as a proficient running buffer for Protein A/G chromatography, facilitating antibody purification based on specific antigen binding. In hematological and biochemical procedures, PBS plays a pivotal role in the effective washing of cells and tissues. In summary, PBS stands out as an indispensable tool in both biological and biochemical research, offering isotonicity, buffering capacity, simplicity, and stability. Its diverse applications in cell biology, reagent preparation, protein purification, and various biological assays underscore its profound significance in advancing scientific knowledge and understanding.
Expanding beyond cell biology, PBS demonstrates versatility in applications such as creating custom ELISA buffers for optimal antibody-antigen interactions. Moreover, it serves as a proficient running buffer for Protein A/G chromatography, facilitating antibody purification based on specific antigen binding. In hematological and biochemical procedures, PBS plays a pivotal role in the effective washing of cells and tissues. In summary, PBS stands out as an indispensable tool in both biological and biochemical research, offering isotonicity, buffering capacity, simplicity, and stability. Its diverse applications in cell biology, reagent preparation, protein purification, and various biological assays underscore its profound significance in advancing scientific knowledge and understanding.
Application
Phosphate buffered saline has been used-
- for the production of platinum nanoparticles (NP-Pt) hydrocolloids[1]
- for the washing of blood sample and SEM (scanning electron microscope) stubs prior to SEM[1]
- for preparing the solution of fluorescent lectin wheat germ agglutinin (WGA)[2]
- for the dilution of capture antibody during ELISA (enzyme linked immunosorbent assay)[3]
- for suspension of cell pellets during centrifugation[3]
- in immunoassay procedures
Features and Benefits
- High-quality product suitable for multiple research applications
- Can be used as a buffer component in biological and biochemical applications
- Highly soluble in water
Reconstitution
One tablet dissolved in 200 mL of deionized water yields 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4, at 25 °C.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
also commonly purchased with this product
recommended
Product No.
Description
Pricing
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
Ensure optimal cell culture outcomes with ultrapure, endotoxin-free water. Learn about the impact of water impurities on mammalian cell cultures and how to achieve optimal cell growth.
Protocols
Antibody purification protocols yield preparations containing endogenous IgG alongside specific antibodies.
Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.
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How much should one tablet weigh for it to yield 200ml 1xPBS? Asking just to make sure we won't use chipped tablets.
1 answer-
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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If I want to make the same buffer molarity as the product P4474-1L, how many tablets should I dissolve in 1L of DI water?
1 answer-
P4417 and P4474 have different components and initial concentrations. One P4417 tablet dissolved in 200 mL of deionized water yields a 0.01 M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M sodium chloride at pH 7.4, 25 °C. P4474 contains 1.058 mM potassium phosphate monobasic, 154 mM sodium chloride, and 2.97 mM sodium phosphate dibasic dihydrate. Due to these differences, it is not feasible to directly match the molarity of P4474 by dissolving P4417 tablets.
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Going by instructions, 1 tab in 200 mL water gives a 1x PBS sol'n. The recipe I use for 1x PBS has the same salt concs. Sorry if this is a silly question, but can I make up a 3x PBS solution simply by dissolving 3 tablets in 200 mL of water?
1 answer-
These tablets have not been tested for solubility or dissolution time at a 3X concentration. The end user would have to determine solubility experimentally. Note that 10X prepared solutions are available and may easily be diluted to the desired concentration. Please see the link below to review several of these product options:
https://www.sigmaaldrich.com/search/pbs-10x?focus=products&page=1&perpage=30&sort=relevance&term=PBS%2010X&type=productHelpful?
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Hi! If with this product I want to do a 10x solution, do i dilute it in 2000 mL or first in 200mL and then do the dilution? Thanks!
1 answer-
Dissolve 1 tablet in 200 ml of water to obtain: 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4 At 25 °C. These instructions are for a 1 X PBS preparation and this product is only intended for this specific concentration. If a 10X concentrate is needed, there are options in the catalog, such as the following products : 79383, P7059, 6506-OP, and 6505-OP.
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Is it possible to obtain the salt components for product 6501-200TAB? Additionally, can you provide the composition details for other PBS tablets or powder products?
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The compositions are as follows:
1. 6501-200TAB: This PBS contains 137mM NaCl, 2.7 mM KCl, and 10mM Na phosphate.
2. P3813 - powder - Packed in Pouch or PAK: Each pouch contains 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride, totaling 9.55 g per packet. This is a standard PBS formulation.
3. P4417- Tablet: When dissolved in 200 mL of deionized water, one tablet yields 0.01 M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M sodium chloride, at pH 7.4 and 25 °C. This product contains the same formulation as P3813 on a per liter basis (excluding tableting fillers): 8 g/L Sodium Chloride, 0.2 g/L Potassium Phosphate, monobasic, 1.15 g/L Sodium Phosphate, dibasic, and 0.2 g/L Potassium Chloride. This mixture should be diluted to one liter of water, resulting in a pH of 7.4, which can be adjusted using phosphoric acid or sodium hydroxide. The concentrations are 10 mM phosphate buffer, 2.7 mM potassium chloride, and 137 mM. These PBS tablets do not contain BSA.
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What is the osmolality of P4417 made to 1X PBS at pH 7.4?
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This PBS once diluted in 200 mL distilled water will account for:
2.7 mM potassium chloride = 5.4 mosmoles / L
137 mM sodium chloride = 274 mosmoles / LThe exact osmolality is not determined.
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Is this PBS calcium-free?
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The formulation of this product is considered proprietary. However, calcium is not included in the composition.
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What is the purity and CAS
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This product is a mixture of components. CAS numbers are not assigned to multi-component products. Likewise, as there are multiple components, purity is not reported. Upon reconstitution according to instructions, the solution will contain 0.01 M phosphate buffer, 0.0027 M potassium chloride, and 0.137 M sodium chloride.
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