- R7647
R7647
Random Nonamers
for use as primers in cDNA synthesis, 50 μM in H2O
Recommended Products
grade
for molecular biology
Quality Level
form
liquid
usage
0.1 mL sufficient for 100 RT-PCR reactions
concentration
50 μM in H2O
shipped in
wet ice
storage temp.
−20°C
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This Item | 11034731001 | SRP0140 | SRP0142 |
|---|---|---|---|
| grade for molecular biology | grade - | grade - | grade - |
| form liquid | form lyophilized | form aqueous solution | form aqueous solution |
| usage 0.1 mL sufficient for 100 RT-PCR reactions | usage sufficient for ≤400 reactions | usage - | usage - |
| concentration 50 μM in H2O | concentration - | concentration >0.02 mg/mL | concentration >0.02 mg/mL |
| shipped in wet ice | shipped in - | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −70°C | storage temp. −70°C |
General description
Application
- First-strand cDNA synthesis
- Preparing cDNA libraries (with oligo (dT) primers
- Prepare labeled DNA probes for hybridizations
Features and Benefits
- When preparing cDNA libraries or dealing with situations where specific primers are not suitable, the random nonamers can be combined with anchored oligo (dT)23 primers (Product No. O4387).
- In cases of incomplete or missing sequence information, or when specific primers are not effective, the random nonamers offer a solution for cDNA library preparation.
- The random nonamers′ priming capability at higher temperatures (up to 65°C) is reduced, ensuring that they do not interfere with polymerase chain reaction(PCR) following transcription.
- Randomnonamers can also be used to prepare labeled DNA probes for hybridizationstudiesRandom nonamers serve as alternatives to specific reverse transcription (RT) primers for first-strand synthesis, cDNA library construction, and other applications.
Components
Other Notes
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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