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Merck

S1638

Streptavidin−Agarose from Streptomyces avidinii

buffered aqueous suspension

Synonym(s):

streptavidin agarose beads, streptavidin agarose resin

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MXP 3,870.00

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MXP 13,107.00

MXP 3,870.00

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About This Item

MDL number:
MFCD00082035
UNSPSC Code:
41106500
NACRES:
NA.56

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form

buffered aqueous suspension

Quality Level

200

extent of labeling

≥1 mg per mL

technique(s)

affinity chromatography: suitable

matrix

4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

7 atoms

capacity

≥15 μg/mL binding capacity (biotin)

storage temp.

2-8°C

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This Item
11093266910S289011089161001
Streptavidin−Agarose from Streptomyces avidinii buffered aqueous suspension

Millipore

S1638

Streptavidin−Agarose from Streptomyces avidinii

Streptavidin-AP-conjugate for nucleic acid detection

Roche

11093266910

Streptavidin-AP-conjugate for nucleic acid detection

Streptavidin−Alkaline Phosphatase from Streptomyces avidinii lyophilized powder, ≥700 DEA units/mg protein

Sigma-Aldrich

S2890

Streptavidin−Alkaline Phosphatase from Streptomyces avidinii

Streptavidin-AP conjugate solution, pkg of 1 mL (1,000U)

Roche

11089161001

Streptavidin-AP conjugate

technique(s)

affinity chromatography: suitable

technique(s)

-

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

100

form

buffered aqueous suspension

form

solution

form

lyophilized powder

form

solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

extent of labeling

≥1 mg per mL

extent of labeling

-

extent of labeling

-

extent of labeling

-

matrix

4% beaded agarose

matrix

-

matrix

-

matrix

-

Application

Streptavidin−agarose from Streptomyces avidinii has been used:
  • to pull down biotinylated cell surface proteins during the quantification of plasma membrane transforming growth factor β (TGFβ) receptor II (TβRII) and Tβ
  • RII internalization
  • in biotinylated miRNA pull-down assay; as secondary antibodies in immunoprecipitation

Streptavidin-agarose is used in protein chromatography, affinity chromatography, and recombinant protein expression and analysis. Streptavidin-agarose has been used to study the oriented immobilization of the tobacco etch virus protease for the cleavage of fusion proteins. Streptavidin-agarose has also been used to develop a method for screening triplex DNA binders from natural plant extracts.
Used for the purification of biotin containing proteins or DNA binding proteins

Biochem/physiol Actions

Streptavidin is a homotetrameric protein, isolated from Streptomyces avidinii, which, like avidin, has a high affinity for biotin. Streptavidin is slightly anionic (pI ~ 5-6) and non-glycosylated. These properties contribute to its relatively low non-specific binding compared to egg white avidin. Streptavidin is also more resistant than avidin to dissociation into subunits by guanidinium chloride. Streptavidin-agarose can be used to immobilize or isolate various biotinylated macromolecules and complexes (proteins, antibodies, lectins, nucleic acids, receptors, and ligands). The inherent high-affinity streptavidin-biotin interaction requires harsh conditions to release biotinylated macromolecules. This feature makes streptavidin-agarose useful in a variety of affinity purification applications.

Physical form

Suspension in 0.01 M sodium phosphate, pH 7.2, containing 0.05 M NaCl and 0.02% sodium azide

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000454452
0000454453
0000454453
0000454452
0000451571

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Behrad Derakhshan et al.
Nature protocols, 2(7), 1685-1691 (2007-07-21)
Covalent addition of nitric oxide (NO) to Cys-sulfur in proteins, or S-nitrosylation, plays pervasive roles in the physiological and pathophysiological modulation of mammalian protein functions. Knowledge of the specific protein Cys residues that undergo NO addition in different biological settings
O Litzka et al.
European journal of biochemistry, 251(3), 758-767 (1998-03-07)
In Aspergillus nidulans, a DNA-binding complex, PENR1, was shown to bind to two CCAAT-box-containing DNA elements located in the promoter regions of the bidirectionally oriented penicillin biosynthesis genes acvA and ipnA, and of the aat promoter. Here, partial purification of
Jonathan A Roberts et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 27(15), 4072-4082 (2007-04-13)
P2X receptors for extracellular ATP are a distinct family of ligand-gated cation channels involved in physiological processes ranging from synaptic transmission to muscle contraction. Common ATP binding motifs are absent from P2X receptors, and the extent of the agonist binding
Niusheng Xu et al.
Analytical chemistry, 84(5), 2562-2568 (2012-01-10)
A novel ligand fishing assay was established to screen triplex DNA binders from complicated samples by a combination of immobilization of triplex DNA on agarose beads and high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). The biotinylated oligodeoxynucleotides were first bound to
I Gottschalk et al.
European journal of biochemistry, 267(23), 6875-6882 (2000-11-18)
Two cytochalasin B-binding states of the human red blood cell facilitative glucose transporter GLUT1 were studied, one exhibiting one cytochalasin B-binding site on every second GLUT1 monomer (state 1) and the other showing one site per monomer (state 2). Quantitative
View All Related Papers

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Product Information Sheet - S1638
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Questions

  1. Do the streptavidin beads have disulphide bonds between the dimers?

    1 answer

    1. No analysis of the post-conjugation structure of streptavidin has been performed. This product is tested for binding capacity and functionality. The product S4762 is used in the production of the S1638.

      See the link below for additional information on S4762, streptavidin, including structure details and references:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/424/896/s4762pis.pdf

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