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Merck

S5897

Sephadex® G-50

BioReagent, DNA grade, Molecular Biology, fine

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25 G

MXP 7,324.00

MXP 7,324.00

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About This Item

CAS Number:
9004-54-0
MDL number:
MFCD23701222.obs1210407
UNSPSC Code:
23151817
NACRES:
NA.52

Key Documents

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grade

Molecular Biology

Quality Level

200

product line

BioReagent

form

beads

technique(s)

DNA purification: suitable

matrix

cross-linked dextran

swelling

1 g swells to 9-11 mL

bead diameter

20-80 μm

pore size

exclusion limit (20 bp dsDNA (rA)20)

pH

2-10

foreign activity

DNase, RNase, none detected

storage temp.

room temp

SMILES string

O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChI key

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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1 of 4

This Item
GE17-0045-02GE17-0573-01GE17-0573-02
Sephadex® G-50 BioReagent, DNA grade, Molecular Biology, fine

Sigma-Aldrich

S5897

Sephadex® G-50

Sephadex® G-50 M DNA grade

GE17-0045-02

Sephadex® G-50

Sephadex® G-50 DNA grade, Cytiva, pack of 25 g

GE17-0573-01

Sephadex® G-50

Sephadex® G-50 Cytiva, pkg of (pack of 100 g)

GE17-0573-02

Sephadex® G-50

grade

DNA grade, for molecular biology

grade

-

grade

DNA grade

grade

DNA grade

technique(s)

DNA purification: suitable

technique(s)

-

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

-

form

beads

form

-

form

-

form

-

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

-

product line

BioReagent

product line

-

product line

-

product line

-

General description

Sephadex® G-50, Fine is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Fine” indicates a small particle size known for excellent efficiency and short diffusion distances.

S5897-100G′s updated product number is GE17-0573-02

Application

Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing to improve the sequencing quality.[1]

Features and Benefits

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000487060
0000487060
0000448099
0000448099
0000376987

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Torben Riehl et al.
PloS one, 7(11), e49354-e49354 (2012-11-13)
The Amundsen Sea, Antarctica, is amongst the most rapidly changing environments of the world. Its benthic inhabitants are barely known and the BIOPEARL 2 project was one of the first to biologically explore this region. Collected during this expedition, Macrostylis
Aron Fazekas et al.
BioTechniques, 48(4), 277-285 (2010-06-24)
Stutter products are a common artifact in the PCR amplification of frequently used genetic markers that contain mononucleotide simple sequence repeats. Despite the importance of accurate determination of nucleotide sequence and allele size, there has been little progress toward decreasing
Mark Del Campo et al.
Journal of molecular biology, 389(4), 674-693 (2009-04-28)
The DEAD-box proteins CYT-19 in Neurospora crassa and Mss116p in Saccharomyces cerevisiae are broadly acting RNA chaperones that function in mitochondria to stimulate group I and group II intron splicing and to activate mRNA translation. Previous studies showed that the
Ryo Takeuchi et al.
Nucleic acids research, 37(3), 877-890 (2008-12-24)
The LAGLIDADG homing endonuclease (LHE) I-AniI has adopted an extremely efficient secondary RNA splicing activity that is beneficial to its host, balanced against inefficient DNA cleavage. A selection experiment identified point mutations in the enzyme that act synergistically to improve
Katharina Besemer et al.
Applied and environmental microbiology, 75(22), 7189-7195 (2009-09-22)
Streams are highly heterogeneous ecosystems, in terms of both geomorphology and hydrodynamics. While flow is recognized to shape the physical architecture of benthic biofilms, we do not yet understand what drives community assembly and biodiversity of benthic biofilms in the
View All Related Papers

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