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SHCLND

MISSION® shRNA

Purified DNA

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About This Item

UNSPSC Code:
41106609
To order shRNA please search for your gene of interest.
Order By Gene

Quality Level

200

shipped in

dry ice

storage temp.

−20°C

General description

Our comprehensive shRNA product offering consists of over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes. The library is available in: gene family sets: gene collections related to specific functional classes such as kinases and ion channels; gene taget sets, sets of shRNA clones that target your favorite gene; and individual shRNA clones. Our shRNA and lentiviral manufacturing platforms utilize dedicated laboratories featuring state-of-the-art robotics, liquid handling and laboratory information management systems (LIMS) to provide superior quality and service for your RNAi research needs.
MISSION® TRC shRNA purified plasmid DNA format takes the hassle out of preparing DNA from each construct. DNA may be used directly for transient or stable transfection. Additionally these plasmids can be combined with packaging plasmids in a packaging cell line to produce lentiviral particles. 1 μg of DNA is provided for each construct in the target set.

Application

MISSION® shRNA has been used:
  • in the generation of ZO-1 (zonula occludens-1) knockdown
  • to knock down LIF (leukemia inhibitory factor) expression in isogenic MCF10 model
  • to knock down nuclear factor erythroid 2 like 1, in n MIN6 pancreatic β-cells

Other Notes

To see protocols and application data please visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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storage temp.

−20°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−20°C

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Still not finding the right product?

Explore all of our products under MISSION® shRNA

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

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Articles

MISSION® Lentiviral shRNA

MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.

Efficient Gene Silencing with MISSION® TRC shRNA

RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.

Related Content

shRNAs for Individual Genes

Find shRNAs for Individual Genes. Over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes.

Deficiency of long isoforms of Nfe2l1 sensitizes MIN6 pancreatic beta cells to arsenite-induced cytotoxicity
Cui Q, et al.
Toxicology and Applied Pharmacology, 329(2), 67-74 (2017)
Epigenetic up-regulation of leukemia inhibitory factor (LIF) gene during the progression to breast cancer
Shin JE, et al.
Molecules and Cells, 31(2), 181-189 (2011)
Eva Ramos-Fernández et al.
Scientific reports, 11(1), 7395-7395 (2021-04-03)
Wnt signaling plays a key role in neurodevelopment and neuronal maturation. Specifically, Wnt5a stimulates postsynaptic assemblies, increases glutamatergic neurotransmission and, through calcium signaling, generates nitric oxide (NO). Trying to unveil the molecular pathway triggering these postsynaptic effects, we found that
View All Related Papers

Global Trade Item Number

SKUGTIN
SHCLND04061835574056

Questions

  1. How is the knockdown measured for validated shRNA clones?

    1 answer

    1. SYBR Green qPCR is utilized to determine the relative silencing effect of specific shRNAs. The term "validated" refers to shRNA sequences that have been tested and confirmed effective in silencing. This validation involves testing in specific cell lines and measuring the mean knockdown level through lentiviral transduction. The evaluation of knockdown is conducted by measuring mRNA transcript levels post-lentiviral transduction with SYBR Green qPCR and comparing these levels to those achieved with the MISSION pLKO.1-puro Control Transduction Particles (SHC001V). For instance, a mean knockdown level of 0.90 indicates a 90% reduction at the mRNA level. Typically, validation does not extend to the protein level, as the primary target of shRNA is mRNA expression, which subsequently influences protein expression.

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