I have extensively used these lentiviral vectors to perform KD experiments, and they work. We have performed experiments in different cell types, from macrophages to epithelial cells, from both mice and humans. I totally recommend it.
SHCLNV
MISSION® shRNA
Lentiviral Particles
MISSION® shRNA
1 of 2 reviewers received a sample product or took part in a promotion
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About This Item
UNSPSC Code:
41106609
Technique(s):
capture ELISA: 106 VP/mL using p24
To order shRNA please search for your gene of interest.
General description
Our comprehensive shRNA product offering consists of over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes. The library is available in: gene family sets: gene collections related to specific functional classes such as kinases and ion channels; gene taget sets, sets of shRNA clones that target your favorite gene; and individual shRNA clones. Our shRNA and lentiviral manufacturing platforms utilize dedicated laboratories featuring state-of-the-art robotics, liquid handling and laboratory information management systems (LIMS) to provide superior quality and service for your RNAi research needs.
Lentiviral particles are ideal for transducing a wide range of cell lines. VSV-G Psuedotyping of the MISSION® lentiviral particles allows for entry into a wide range of cells. In addition, lentiviral particles stably integrate the shRNA into the genomes of both dividing and non-dividing cell lines. The MISSION bioproduction team regularly produces high quality lentivirus, so that our customers can get straight to their RNAi experiment without wasting precious time on tedious lentiviral production. Our minimum titer is 106 VP/mL. Unlike adenoviral infections that require vast excesses, lentiviral transduction is efficient enought allow for MOI as low as 1.
Application
MISSION® shRNA has been used:
- in transfections and transductions
- in the establishment of stable cell lines expressing shRNA
- in the generation of alpha-actinin-4 (ACTN4) knockdown cell line
- to transduce raw 264.7 cells[1]
Other Notes
To see protocols and application data please visit sigma.com/shrna.
Legal Information
Use of this product is subject to one or more license agreements.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| technique(s) capture ELISA: 106 VP/mL using p24 | technique(s) capture ELISA: 106 VP/mL using p24 | technique(s) - | technique(s) capture ELISA: 5 x 108 TU/mL using p24 |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −70°C | storage temp. −70°C | storage temp. −20°C | storage temp. −70°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
FACS sorts cells based on light scattering and fluorescence for objective cell analysis.
Articles
RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.
Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.
Related Content
Find shRNAs for Individual Genes. Over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes.
Instructions
Vera Mugoni et al.
Cell research, 29(6), 446-459 (2019-04-27)
Although targeted therapies have proven effective and even curative in human leukaemia, resistance often ensues. IDH enzymes are mutated in ~20% of human AML, with targeted therapies under clinical evaluation. We here characterize leukaemia evolution from mutant IDH2 (mIDH2)-dependence to
Barbara D Boyan et al.
Scientific reports, 8(1), 8588-8588 (2018-06-07)
Successful osseointegration of an endosseous implant involves migration and differentiation of mesenchymal stem cells (MSCs) on the implant surface. Micro-structured, hydrophilic titanium surfaces direct MSCs to undergo osteoblastic differentiation in vitro, in the absence of media additives commonly used in
alpha-Actinin-4 confers radioresistance coupled invasiveness in breast cancer cells through AKT pathway
Desai S, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1865(1), 196-208 (2018)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SHCLNV | 04061838262202 |