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SRP3085

Sigma-Aldrich

IL-2 human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):
T-cell growth factor (TCGF), Aldesleukin
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.1 ng/mL ED50

mol wt

15.5 kDa

packaging

pkg of 50 μg

application(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL2(3558)

General description

IL-2 (interleukin 2) is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is a type I cytokine. The gene is mapped to human chromosome 4q27. Recombinant human IL-2 is a 15.5kDa protein, containing 134 amino acid residues including one intrachain disulfide bond.

Application

  • IL-2 (interleukin 2) human has been used for the detection of IL-2 using 4-Fluoro-3-nitrophenyl grafted gold electrode based platform.
  • It has been used for the production of tumor-specific cytotoxic T lymphocytes.
  • It has been used for the proliferation of T lymphocytes.

Biochem/physiol Actions

IL-2/IL-2R (interleukin 2/IL-2 receptor) signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK (natural killer) cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. It is associated with recurrent oral ulcer and asthma and Mycoplasma pneumoniae infection in children. Absence of IL2 in mice causes fatal lymphoproliferative inflammatory disease (IL-2 deficiency syndrome). IL-2 signaling involves three pathways: phosphoinositol 3-kinase (PI3-K)/AKT, Ras-MAPK (mitogen activated protein kinase), and JAK-STAT (Janus kinase - signal transducers and activators of transcription) pathways.

Sequence

MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in 100mM Acetic Acid to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

The common gamma-chain cytokines IL-2, IL-7, IL-15, and IL-21 induce the expression of programmed death-1 and its ligands.
Kinter AL
Journal of Immunology, 181, 6738-6746 (2008)
4-Fluoro-3-nitrophenyl grafted gold electrode based platform for label free electrochemical detection of interleukin-2 protein.
Arya SK and Park MK
Biosensors And Bioelectronics, 61, 260-265 (2014)
A mechanism of hypoxia-mediated escape from adaptive immunity in cancer cells.
Barsoum IB
Cancer Research, 74, 665-674 (2014)
Prostaglandin E2 (PGE2)-dependent suppression of interleukin alpha (IL-2) production in patients with major trauma.
Faist E
The Journal of Trauma, 27, 837-848 (1987)
Family based association analysis of the IL2 and IL15 genes in allergic disorders.
Christensen U
European Journal of Human Genetics, 14, 227-235 (2006)

Protocols

Protocol Guide: WST-1 Assay for Cell Proliferation and Viability

WST-1 assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for WST-1 reagent preparation and examples of applications. Frequently asked questions and troubleshooting guide for WST-1 assay.

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