SRP3267

NT-3 human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(s): HGNF, NGF-2, Nerve growth factor-2
• NACRES: NA.32
• UNSPSC Code: 12352202

Properties

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC), ≥98% (SDS-PAGE)
• form: lyophilized
• potency: 20-50 ng/mL ED₅₀
• mol wt: 27.2 kDa
• packaging: pkg of 10 μg
• impurities: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• UniProt accession no.: P20783
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... NTF3(4908)

Description

Biochem/physiol Actions

NT3 functions on two structurally unrelated receptor types- the p75 neurotrophin receptor (p75^NTR) and the receptor tyrosine kinase (TRK). It promotes the metastatic invasion of brain by breast cancer cells, by promoting the EMT (epithelial-mesenchymal transition) transition of breast cancer cells and suppressing the immune response in brain.[1] This protein is responsible for the indction and survival of neurite outgrowth from a subset of neural crest (NC) and placode-derived neurons.[2]
Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kDa polypeptide monomers (240 total amino acid residues).

General description

NTF3 (neurotrophin 3) is a neurotrophic factor belonging to the NGF (nerve growth factor) family.[1] This protein is composed of 257 amino acids, with a prepro region of 138 amino acids and the mature sequence of 119 residues.[3]

Physical form

Lyophilized with no additives.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable