T0665

Sigma-Aldrich

holo-Transferrin human

powder, BioReagent, suitable for cell culture, ≥97%

Synonym(s):
Siderophilin, iron-saturated
CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

Quality Level

biological source

human plasma

description

Iron-saturated

product line

BioReagent

assay

≥97%

form

powder

application(s)

cell culture | mammalian: suitable

impurities

HIV and HBsAg, none detected
endotoxin, tested

solubility

H2O: 50 mg/mL

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... TF(7018)

Looking for similar products? Visit Product Comparison Guide

Application

Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human holo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Human holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.
holo-Transferrin human has been supplemented in Roswell Park Memorial Institute (RPMI) for proerythroblasts proliferation assay and also in culture media for inductively coupled plasma emission spectroscopy.

Packaging

50, 100, 500 mg in poly bottle
1 g in poly bottle

Biochem/physiol Actions

Provides iron to cells in a physiologically stable and safe form.

Preparation Note

Heat treated at 60 °C (minimum) for a minimum of 10 hours.

Analysis Note

Purity by agarose gel electrophoresis.
Iron content by ICP reported on a lot-specific basis.

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 3

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the carbohydrate content of human transferrin?

    We have found the information on transferrin in a reference book:The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II.According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965).The table also gives information on the percentage of each type of monosaccharide that is present.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Co-operative signalling mechanisms required for erythroid precursor expansion in response to erythropoietin and stem cell factor
Arcasoy O and Jiang X
British Journal of Haematology, 130(1), 121-129 (2005)
Jill M Brown et al.
Nature communications, 9(1), 3849-3849 (2018-09-23)
Self-interacting chromatin domains encompass genes and their cis-regulatory elements; however, the three-dimensional form a domain takes, whether this relies on enhancer-promoter interactions, and the processes necessary to mediate the formation and maintenance of such domains, remain unclear. To examine these...
Pamela Sarkar et al.
Cytotherapy, 20(1), 21-28 (2017-09-18)
Clinical trials using ex vivo expansion of autologous mesenchymal stromal cells (MSCs) are in progress for several neurological diseases including multiple sclerosis (MS). Given that environment alters MSC function, we examined whether in vitro expansion, increasing donor age and progressive...
Engineered mitochondrial ferritin as a magnetic resonance imaging reporter in mouse olfactory epithelium
Iordanova B, et al.
Testing, 8(8), e72720-e72720 (2013)
Senthil Raja Jayapal et al.
Cell cycle (Georgetown, Tex.), 15(22), 3070-3081 (2016-09-23)
Cyclin A2 is an essential gene for development and in haematopoietic stem cells and therefore its functions in definitive erythropoiesis have not been investigated. We have ablated cyclin A2 in committed erythroid progenitors in vivo using erythropoietin receptor promoter-driven Cre...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service