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T2076

Sigma-Aldrich

TargeTron Vector pAR1219

Expression Vector for Bacterial Gene Knockout

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shipped in

wet ice

storage temp.

−20°C

General description

Plasmid pAR1219 is a pBR322-based vector that expresses T7 RNA Polymerase under control of the IPTG inducible lacUV5 promoter and is intended for use with the TargeTron Gene Knockout System (TA0100).

Application

Many TargeTron system plasmids use the T7 promoter for intron expression. By co-transforming plasmid pAR1219 with the TargeTron pACD4 plasmids, the T7 promoter can be used to express the intron and disrupt chromosomal genes in alternative hosts such as Salmonella typhimurium and Shigella flexneri. Chromosomal gene disruptions in non-DE3 strains of E. coli can also be performed using pAR1219 with the pACD4 intron expression plasmids. TargeTron Vector pAR1219 has been used for the overexpression of T7 RNA polymerase in the preparation of S30-T7 lysate. It has also been used to transform MRE600 E. coli strains.

Preparation Note

To use pAR1219 in conjunction with the pACD4 plasmids, simply co-transform both plasmids and select in a liquid medium containing: 50 mg/ml ampicillin, 25 mg/ml chloramphenicol, and 1% glucose. Glucose is typically included to provide additional suppression of the lac UV5 promoter prior to IPTG-induction.

Other Notes

For more information and to view applications data, please visit www.sigma-aldrich.com/targetron.

Legal Information

TargeTron is a trademark of InGex, LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Group II introns as controllable gene targeting vectors for genetic manipulation of bacteria.
Karberg M, et al.
Nature Biotechnology, 19(12), 1162-1162 (2001)
A simple and rapid method for preparing a cell-free bacterial lysate for protein synthesis.
Krinsky N, et al.
PLoS ONE, 11(10), e0165137-e0165137 (2016)
Synthetic Cells Synthesize Therapeutic Proteins inside Tumors.
Krinsky N, et al.
Advanced Helathcare Materials (2017)

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