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T3253

Sigma-Aldrich

Trizma® hydrochloride

reagent grade, ≥99.0% (titration), crystalline

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Synonym(s):
TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
Beilstein:
3675235
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

reagent grade

Quality Level

Assay

≥99.0% (titration)

form

crystalline

storage condition

dry at room temperature

technique(s)

affinity chromatography: suitable

impurities

≤0.5% water (Karl Fischer)

color

white

useful pH range

7.0-9.0

pKa (25 °C)

8.1

mp

150-152 °C

solubility

H2O: soluble, clear, colorless

density

1.39 g/cm3 at 20—25 °C
1.41 g/cm3 at 20—25 °C

absorption

≤0.05 at 290 nm at 40%

suitability

suitable for mass spectrometry

application(s)

diagnostic assay manufacturing
life science and biopharma
sample preparation

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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General description

Tris HCl, or Trizma® hydrochloride, is a widely utilized acidic buffer in the physiological pH range of 7.3 to 7.5. With a pKa of 8.08 at 25°C, it offers a pH range of 7.0 to 9.0, aligning with living organisms′ typical pH. Essential in various biological, cell biology, and biochemistry applications, Tris HCl is crucial for effective pH control in buffer solutions. Notably, Tris base and Tris hydrochloride alone lack sufficient buffering capacity, prompting their combination within the pH range of 7 to 9.

Tris buffers play a key role in techniques like protein electrophoresis and western blotting, serving in low ionic strength buffers for protein transfer. They are also integral to SDS-PAGE gels, running buffers, and blotting buffers. Additionally, Tris salts, especially in TAE and TBE buffers, are extensively employed in DNA agarose electrophoresis. These buffers are pivotal in diverse applications, including biochemistry, protein purification, and electrophoresis, showcasing the versatility and importance of Tris HCl in maintaining optimal pH for biochemical and cell biology research.

Application

Trizma® hydrochloride has been used as a buffer solution for staining procedure, reduction and alkylation studies and in situ hybridization procedure.­

Features and Benefits

  • Ideal as a Biological buffer for Cell Biology and Biochemical research
  • Effective Buffering from pH 7-9 with a pKa of 8.1 (25 °C)
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications


Other Notes

For additional information on our range of Biochemicals, please complete this form.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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