T5516

Sigma-Aldrich

3,3′,5-Triiodo-L-thyronine sodium salt

γ-irradiated, powder, suitable for, suitable for cell culture

Synonym(s):
Liothyronine, O-(4-Hydroxy-3-iodophenyl)-3,5-diiodo-L-tyrosine sodium salt, T3
Empirical Formula (Hill Notation):
C15H11I3NNaO4
CAS Number:
Molecular Weight:
672.96
Beilstein/REAXYS Number:
8179867
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.77

Quality Level

biological source

synthetic (organic)

sterility

γ-irradiated

assay

≥90% (HPLC)

form

powder

application(s)

cell culture | mammalian: suitable

suitability

suitable for

shipped in

ambient

storage temp.

−20°C

SMILES string

[Na+].N[C@@H](Cc1cc(I)c(Oc2ccc(O)c(I)c2)c(I)c1)C([O-])=O

InChI

1S/C15H12I3NO4.Na/c16-9-6-8(1-2-13(9)20)23-14-10(17)3-7(4-11(14)18)5-12(19)15(21)22;/h1-4,6,12,20H,5,19H2,(H,21,22);/q;+1/p-1/t12-;/m0./s1

InChI key

SBXXSUDPJJJJLC-YDALLXLXSA-M

Gene Information

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General description

3,3′,5′-Triiodo-L-thyronine (T3) is an endocrine hormone produced by the thyroid gland and is regarded as an active thyroid hormone at cellular level. Thyroxine (T4) serves as a precursor for T3 and is generated in extrathyroidal tissues.

Application

3,3′,5-Triiodo-L-thyronine sodium salt has been used:
  • as a supplement in colon organ culture media
  • to stimulate HuH-7 cells for analysing the hormonal influence on MBL synthesis
  • to study its effect on human growth hormone (GH) versus mouse GH expression in the presence of ′′intact′′ GH genes and in a common pituitary cell context

Biochem/physiol Actions

3,3′,5′-Triiodo-L-thyronine (T3) is a thyroid hormone that increases rates of proteins synthesis, stimulates the breakdown of cholesterol, and effects embryonic development. In cell culture, T3 regulates cell differentiation and protein expression. The interaction of T3 with nonhistone proteins in the chromatin initiates the thyroid hormone activity. Thyroid hormones regulate the differentiation and development of tissues and affect a number of metabolic processes. The level of circulating T3 hormone is reduced in hypothyroidism.

Physical form

powder-0 °C; stock-frozen in working aliquots, avoid repeated freeze/thaw, solution stable 2-8 °C, 30 days

Preparation Note

Package size based on 3,3′,5-Triiodo-L-thyronine sodium salt content; vial may also contain inert salts, such as sodium chloride

Reconstitution

To prepare 20 μg/ml stock solution: add 1 ml 1 N NaOH; gently swirl to dissolve; add 49 ml sterile medium.

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Handbook of Behavior, Food and Nutrition, 2109-2109 (2011)
C M Sørensen et al.
Clinical and experimental immunology, 145(1), 173-182 (2006-06-24)
Activation of the complement system via the plasma protein mannan-binding lectin (MBL) provides a first line of defence against infections. The plasma level of MBL is, in part, determined genetically, but may also be influenced by different hormones in vivo....
Transgenic mice expressing the human growth hormone gene provide a model system to study human growth hormone synthesis and secretion in non-tumor-derived pituitary cells: differential effects of dexamethasone and thyroid hormone
Vakili H, et al.
Molecular and cellular endocrinology, 345(1-2), 48-57 (2011)
Medical Biochemistry, 769-769 (2001)
Human colon tissue in organ culture: preservation of normal and neoplastic characteristics
Dame MK, et al.
In Vitro Cellular & Developmental Biology. Animal, 46(2), 114-122 (2010)
Articles
Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.
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