T8158

Transferrin human

powder, BioReagent, suitable for cell culture

• Synonym(s): Siderophilin, partially saturated
• CAS Number: 11096-37-0
• EC Number: 234-318-8
• MDL number: MFCD00132442
• NACRES: NA.75

Properties

• product line: BioReagent
• Quality Level: 200
• Assay: ≥98%
• form: powder
• concentration: ~25 mM
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested; hepatitis and HIV antibody, none detected
• solubility: H₂O: 50 mg/mL
• UniProt accession no.: P02787
• shipped in: ambient
• storage temp.: −20°C
• Gene Information: human ... TF(7018)

Description

General description

Transferrin is a glycoprotein with a branched carbohydrate chain, having two sites for ferric ion binding in its polypeptide chain.

Application

Transferrin human has been used:

• to incubate human proximal tubule cells (HKC) - 8 cells along with IgG for the examination of albumin specificity
• as a supplement in serum-containing/devoid medium for human thyroid cell culture
• as a target glycoprotein to develop an in-gel hydrazinolysis method

Biochem/physiol Actions

Transferrin mediates iron transportation from plasma to cells. The iron-bound transferrin are of three forms: monoferric A/B transferrin and diferric transferrin. The transferrin binds to specific cell surface receptors and are internalized by endocytosis. Once inside, the iron is released into the cells and the iron-free transferrin, also referred to as apotransferrin, is exocytosed outside the cell without being degraded. Transferrin is known to deliver iron to cells in a physiologically safe and effective form.

Other Notes

Partially iron-saturated

Preparation Note

Heat treated at 60 °C for ten hours.

Analysis Note

Purity by agarose electrophoresis.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable